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1994 1
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Page 1
Leloir glycosyltransferases of natural product C-glycosylation: structure, mechanism and specificity.
Tegl G, Nidetzky B. Tegl G, et al. Biochem Soc Trans. 2020 Aug 28;48(4):1583-1598. doi: 10.1042/BST20191140. Biochem Soc Trans. 2020. PMID: 32657344 Review.
Despite sharing basic evolutionary relationships, as evidenced by their common membership to glycosyltransferase family GT-1 and conserved GT-B structural fold, the known C-glycosyltransferases are diverse in the structural features that govern their reactivity, selectivit …
Despite sharing basic evolutionary relationships, as evidenced by their common membership to glycosyltransferase family GT-1 and conserved G …
Structure-function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferases.
Goedl C, Griessler R, Schwarz A, Nidetzky B. Goedl C, et al. Biochem J. 2006 Aug 1;397(3):491-500. doi: 10.1042/BJ20060029. Biochem J. 2006. PMID: 16640506 Free PMC article.
Unlike the natural phosphorylase that was previously obtained as a truncated 61 kDa monomer containing one tightly bound Mg2+, the intact enzyme produced in E. coli is a dimer and not associated with metal ions [Eis, Watkins, Prohaska and Nidetzky (2001) Biochem. J. 356, 7 …
Unlike the natural phosphorylase that was previously obtained as a truncated 61 kDa monomer containing one tightly bound Mg2+, the intact en …
Relationships between structure, function and stability for pyridoxal 5'-phosphate-dependent starch phosphorylase from Corynebacterium callunae as revealed by reversible cofactor dissociation studies.
Griessler R, Psik B, Schwarz A, Nidetzky B. Griessler R, et al. Eur J Biochem. 2004 Aug;271(16):3319-29. doi: 10.1111/j.1432-1033.2004.04265.x. Eur J Biochem. 2004. PMID: 15291809 Free article.
The inactivation rate of CcStP under resolution conditions at 30 degrees C was, respectively, four- and threefold reduced in two mutants, Arg234-->Ala and Arg242-->Ala, previously shown to cause thermostabilization of CcStP [Griessler, R., Schwarz, A., Mucha, J. & Ni …
The inactivation rate of CcStP under resolution conditions at 30 degrees C was, respectively, four- and threefold reduced in two mutants, Ar …
Energetics of the Glycosyl Transfer Reactions of Sucrose Phosphorylase.
Vyas A, Nidetzky B. Vyas A, et al. Biochemistry. 2023 Jun 20;62(12):1953-1963. doi: 10.1021/acs.biochem.3c00080. Epub 2023 May 30. Biochemistry. 2023. PMID: 37253063 Free PMC article.
Enzyme glycosylation from sucrose and alpha-glucose 1-phosphate (Glc1P) is rate-limiting in the forward (k(cat) = 84 s(-1)) and reverse direction (k(cat) = 22 s(-1)) of reaction at 30 C. Enzyme-substrate association is driven by entropy (TdeltaS(b) +23 kJ/mol), likely aris …
Enzyme glycosylation from sucrose and alpha-glucose 1-phosphate (Glc1P) is rate-limiting in the forward (k(cat) = 84 s(-1)) and reverse dire …
Mechanistic differences among retaining disaccharide phosphorylases: insights from kinetic analysis of active site mutants of sucrose phosphorylase and alpha,alpha-trehalose phosphorylase.
Goedl C, Schwarz A, Mueller M, Brecker L, Nidetzky B. Goedl C, et al. Carbohydr Res. 2008 Aug 11;343(12):2032-40. doi: 10.1016/j.carres.2008.01.029. Epub 2008 Feb 2. Carbohydr Res. 2008. PMID: 18346723 Review.
Mutagenesis studies suggested a putative active-site structure for this trehalose phosphorylase that is typical of retaining glycosyltransferases of fold family GT-B and markedly different from that of sucrose phosphorylase. While ambiguity remains regarding the chemical m …
Mutagenesis studies suggested a putative active-site structure for this trehalose phosphorylase that is typical of retaining glycosyltransfe …
Chemoenzymatic Syntheses of Fluorine-18-Labeled Disaccharides from [18F] FDG Yield Potent Sensors of Living Bacteria In Vivo.
Sorlin AM, López-Álvarez M, Rabbitt SJ, Alanizi AA, Shuere R, Bobba KN, Blecha J, Sakhamuri S, Evans MJ, Bayles KW, Flavell RR, Rosenberg OS, Sriram R, Desmet T, Nidetzky B, Engel J, Ohliger MA, Fraser JS, Wilson DM. Sorlin AM, et al. J Am Chem Soc. 2023 Aug 16;145(32):17632-17642. doi: 10.1021/jacs.3c03338. Epub 2023 Aug 3. J Am Chem Soc. 2023. PMID: 37535945 Free PMC article.
27 results