qDSB-Seq is a general method for genome-wide quantification of DNA double-strand breaks using sequencing.
Zhu Y, Biernacka A, Pardo B, Dojer N, Forey R, Skrzypczak M, Fongang B, Nde J, Yousefi R, Pasero P, Ginalski K, Rowicka M.
Zhu Y, et al.
Nat Commun. 2019 May 24;10(1):2313. doi: 10.1038/s41467-019-10332-8.
Nat Commun. 2019.
PMID: 31127121
Free PMC article.
We induce spike-in DSBs by a site-specific endonuclease and use them to quantify detected DSBs (labeled, e.g., using i-BLESS). Utilizing qDSB-Seq, we determine numbers of DSBs induced by a radiomimetic drug and replication stress, and reveal two orders of magnitude …
We induce spike-in DSBs by a site-specific endonuclease and use them to quantify detected DSBs (labeled, e.g., using i-BLESS). …