Molecular recognition of sulfotyrosine and phosphotyrosine by the Src homology 2 domain

Tong Ju; Wei Niu; Ronald Cerny; Joshua Bollman; Anthony Roy; Jiantao Guo

doi:10.1039/c3mb70061e

Molecular recognition of sulfotyrosine and phosphotyrosine by the Src homology 2 domain

Mol Biosyst. 2013 Jul;9(7):1829-32. doi: 10.1039/c3mb70061e. Epub 2013 Apr 9.

Authors

Tong Ju¹, Wei Niu, Ronald Cerny, Joshua Bollman, Anthony Roy, Jiantao Guo

Affiliation

¹ Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.

PMID: 23567872
DOI: 10.1039/c3mb70061e

Abstract

We study molecular recognition of two closely resembling posttranslational modification products, phosphotyrosine (pTyr) and sulfotyrosine (sTyr). The differences in charge, size, and binding geometry of pTyr and sTyr are the main recognition elements. Protein engineering can be applied to alter the specificity and strength of the recognition.

MeSH terms

Amino Acid Sequence
Humans
Models, Molecular
Molecular Sequence Data
Phosphotyrosine / chemistry*
Phosphotyrosine / metabolism
Protein Binding
Protein Conformation
STAT1 Transcription Factor / chemistry
STAT1 Transcription Factor / metabolism
Sequence Alignment
Tyrosine / analogs & derivatives*
Tyrosine / chemistry
Tyrosine / metabolism
src Homology Domains / physiology*

Substances

STAT1 Transcription Factor
Phosphotyrosine
tyrosine O-sulfate
Tyrosine