Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies

Isaac Vizcaino-Caston; Catherine A Kelly; Annabel V L Fitzgerald; Gary A Leeke; Mike Jenkins; Tim W Overton

doi:10.1016/j.jbiosc.2015.04.021

Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies

J Biosci Bioeng. 2016 Jan;121(1):101-104. doi: 10.1016/j.jbiosc.2015.04.021. Epub 2015 Jul 2.

Authors

Isaac Vizcaino-Caston¹, Catherine A Kelly², Annabel V L Fitzgerald², Gary A Leeke³, Mike Jenkins², Tim W Overton⁴

Affiliations

¹ School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK; Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
² School of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
³ School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
⁴ School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK; Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. Electronic address: t.w.overton@bham.ac.uk.

PMID: 26143034
DOI: 10.1016/j.jbiosc.2015.04.021

Abstract

We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using Fourier transform infrared spectroscopy and differential scanning calorimetry.

Keywords: Bioplastic; Dimethyl sulphoxide; Polyhydroxyalkanoate; Solvent extraction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calorimetry, Differential Scanning
Chloroform / chemistry
Cupriavidus necator / chemistry*
Cupriavidus necator / cytology
Dimethyl Sulfoxide / chemistry*
Polyhydroxyalkanoates / chemistry*
Polyhydroxyalkanoates / isolation & purification*
Solvents / chemistry
Spectroscopy, Fourier Transform Infrared
Time Factors

Substances

Polyhydroxyalkanoates
Solvents
Chloroform
Dimethyl Sulfoxide