Mesocosm experiment to determine the contribution of adsorption, biodegradation, hydrolysis and photodegradation in the attenuation of antibiotics at the water sediment interface

To understand the fate of antibiotics in the aquatic environment, we need to evaluate to which extent the following processes contribute to the overall antibiotic attenuation: adsorption to river sediment, biodegradation, hydrolysis and photodegradation. A laboratory scale mesocosm experiment was conducted in 10 L reactors filled with river sediment and water. The reactors were spiked with four classes of antibiotics (fluoroquinolones, macrolides, sulfonamides, tetracyclines), as well as clindamycin and trimethoprim. The experimental-set-up was designed to study the attenuation processes in parallel in one mesocosm experiment, hence also considering synergetic effects. Our results showed that antibiotics belonging to the same class exhibited similar behavior. Adsorption was the main attenuation process for the fluoroquinolones and tetracyclines (44.4 to 80.0 %). For the sulfonamides, biodegradation was the most frequent process (50.2 to 65.1 %). Hydrolysis appeared to be significant only for tetracyclines (12.6 to 41.8 %). Photodegradation through visible light played a minor role for most of the antibiotics - fluoroquinolones, sulfonamides, and trimethoprim (0.7 to 24.7 %). The macrolides were the only class of antibiotics not affected by the studied processes and they persisted in the water phase. Based on our results, we propose to class the antibiotics in three groups according to their persistence in the water phase. Fluoroquinolones and tetracyclines were non-persistent (half-lives shorter than 11 d). Chlorotetracycline, sulfapyridine and trimethoprim showed a moderate persistence (half-lives between 12 and 35 d). Due to half-lives longer than 36 d sulfonamides and clindamycin were classified as persistent.