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Engineering the surface properties of a human monoclonal antibody prevents self-association and rapid clearance in vivo.
Dobson CL, Devine PW, Phillips JJ, Higazi DR, Lloyd C, Popovic B, Arnold J, Buchanan A, Lewis A, Goodman J, van der Walle CF, Thornton P, Vinall L, Lowne D, Aagaard A, Olsson LL, Ridderstad Wollberg A, Welsh F, Karamanos TK, Pashley CL, Iadanza MG, Ranson NA, Ashcroft AE, Kippen AD, Vaughan TJ, Radford SE, Lowe DC. Dobson CL, et al. Among authors: kippen ad. Sci Rep. 2016 Dec 20;6:38644. doi: 10.1038/srep38644. Sci Rep. 2016. PMID: 27995962 Free PMC article.
Evaluation of strategies to control Fab light chain dimer during mammalian expression and purification: A universal one-step process for purification of correctly assembled Fab.
Spooner J, Keen J, Nayyar K, Birkett N, Bond N, Bannister D, Tigue N, Higazi D, Kemp B, Vaughan T, Kippen A, Buchanan A. Spooner J, et al. Among authors: kippen a. Biotechnol Bioeng. 2015 Jul;112(7):1472-7. doi: 10.1002/bit.25550. Epub 2015 Mar 12. Biotechnol Bioeng. 2015. PMID: 25619171