The ability to identify male-specific DNA renders Y-chromosomal STR systems an invaluable help in cases of rape, other sexual assault as well as in kinship testing. The "PowerPlex Y System" is a recent kit by Promega, that co-amplifies the repeat regions of 11 Y-STRs loci. In the present study, we analyzed the allelic distribution of Y-STRs loci in around 300 unrelated males belonging to the three populations of Calabria region.