Spectrofluorimetric analysis of famotidine in pharmaceutical preparations and biological fluids by derivatization with benzoin

Spectrochim Acta A Mol Biomol Spectrosc. 2015 Jan 5:134:449-52. doi: 10.1016/j.saa.2014.06.118. Epub 2014 Jun 27.

Abstract

A sensitive and simple spectrofluorimetric method has been developed for the analysis of famotidine, from pharmaceutical preparations and biological fluids after derivatization with benzoin. The reaction was carried out in alkaline medium with measurement of fluorescence intensity at 446 nm with excitation wavelength at 286 nm. Linear calibration was obtained with 0.5-15 μg/ml with coefficient of determination (r(2)) 0.997. The factors affecting the fluorescence intensity were optimized. The pharmaceutical additives and amino acid did not interfere in the determination. The mean percentage recovery (n=4) calculated by standard addition from pharmaceutical preparation was 94.8-98.2% with relative standard deviation (RSD) 1.56-3.34% and recovery from deproteinized spiked serum and urine of healthy volunteers was 98.6-98.9% and 98.0-98.4% with RSD 0.34-0.84% and 0.29-0.87% respectively.

Keywords: Benzoin; Deproteinized; Derivatization; Famotidine; Serum and urine.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzoin / chemistry*
  • Famotidine / analysis
  • Famotidine / blood*
  • Famotidine / urine*
  • Histamine H2 Antagonists / analysis
  • Histamine H2 Antagonists / blood*
  • Histamine H2 Antagonists / urine*
  • Humans
  • Limit of Detection
  • Spectrometry, Fluorescence / methods

Substances

  • Histamine H2 Antagonists
  • Famotidine
  • Benzoin