Effects of Dietary Sodium Nitroprusside and N^G-Nitro-L-Arginine Methyl Ester on Growth Performance, Some Organs Development Status and Immune Parameters in Broilers

This study was conducted to determine the effects of dietary supplementation of sodium nitroprusside (SNP), a nitric oxide (NO) donor, and NG-nitro-L-arginine methyl ester (L-NAME), an NO synthase inhibitor, on growth performance, organ development, and immunity in broilers. A total of 560 one-day-old mixed-gender broiler chickens (ROSS 308) were divided into one control and seven experimental groups. The experimental groups were fed a basal diet supplemented with 25, 50, 100, and 200 ppm SNP, and 25, 50, and 100 ppm L-NAME in the starter and grower diets. Body weight gain increased in groups receiving 25-100 ppm L-NAME on day 21 and 100 ppm L-NAME on days 0-42. Feed intake increased in the group receiving 100 ppm L-NAME on all days. The feed conversion ratio improved in the group receiving 25 ppm L-NAME on days 0-21, whereas it worsened in groups with 100 and 200 ppm SNP on days 0-42. Serum antibody titers decreased in the 100 ppm L-NAME group on day 21. In conclusion, the supplementation of the NO synthase inhibitor L-NAME to the broilers' diet had a positive effect on the performance parameters, whereas the NO donor SNP worsened these parameters, especially on days 0-21.