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Multiplexed labeling of cellular proteins with split fluorescent protein tags.
Tamura R, Jiang F, Xie J, Kamiyama D. Tamura R, et al. Commun Biol. 2021 Feb 26;4(1):257. doi: 10.1038/s42003-021-01780-4. Commun Biol. 2021. PMID: 33637968 Free PMC article.
Our multiplexing approach, using the new orthogonal split FP systems, demonstrates simultaneous imaging of four distinct proteins in single cells; the resulting images reveal nuclear localization of focal adhesion protein Zyxin....
Our multiplexing approach, using the new orthogonal split FP systems, demonstrates simultaneous imaging of four distinct proteins in single …
Characterization and development of photoactivatable fluorescent proteins for single-molecule-based superresolution imaging.
Wang S, Moffitt JR, Dempsey GT, Xie XS, Zhuang X. Wang S, et al. Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8452-7. doi: 10.1073/pnas.1406593111. Epub 2014 May 27. Proc Natl Acad Sci U S A. 2014. PMID: 24912163 Free PMC article.
Here, we evaluated these properties for 12 commonly used PAFPs fused to both bacterial target proteins, H-NS, HU, and Tar, and mammalian target proteins, Zyxin and Vimentin. Notably, none of the existing PAFPs provided optimal performance in all four criteria, particularly …
Here, we evaluated these properties for 12 commonly used PAFPs fused to both bacterial target proteins, H-NS, HU, and Tar, and mammalian tar …