Anti-inflammatory effect of trans-anethol in a rat model of myocardial ischemia-reperfusion injury

Marwa Matboli; Amany Helmy Hasanin; Shaimaa Hamady; Eman Khairy; Reham Hussein Mohamed; Yasmin M Aboul-Ela; Mona Hussien Raafat; Sara Abdel Gawad Elsebay; Hossam Y Emam; Rania Shamekh Shamekh; Sara H A Agwa

doi:10.1016/j.biopha.2022.113070

Anti-inflammatory effect of trans-anethol in a rat model of myocardial ischemia-reperfusion injury

Biomed Pharmacother. 2022 Jun:150:113070. doi: 10.1016/j.biopha.2022.113070. Epub 2022 May 6.

Affiliations

¹ Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: DrMarwa_Matboly@med.asu.edu.eg.
² Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: amany_hasanin@med.asu.edu.eg.
³ Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt. Electronic address: shaimaahamady@sci.asu.edu.eg.
⁴ Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: dreman_khairy@med.asu.edu.eg.
⁵ Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: rehamhussein@med.asu.edu.eg.
⁶ Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: dr.yasmin.mahmoud@med.asu.edu.eg.
⁷ Histology and Cell Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: mona.raafat@med.asu.edu.eg.
⁸ Histology and Cell Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: sara_elsebaey@med.asu.edu.eg.
⁹ Anatomy Department, Faculty of Medicine, Cairo University, Egypt. Electronic address: dr_hossamyahia@hotmail.com.
¹⁰ Department of Pathology, University of South Florida, Tampa, FL 33620, USA. Electronic address: Rania.shamekh@gmail.com.
¹¹ Clinical Pathology and Molecular Genomics Unit, Faculty of Medicine, Medical Ain Shams Research Institute (MASRI), Ain Shams University, Cairo, Egypt. Electronic address: sara.h.agwa@med.asu.edu.eg.

PMID: 35658236
DOI: 10.1016/j.biopha.2022.113070

Abstract

Myocardial ischemia‑reperfusion injury (MI/R) is considered a main risk factor for global cardiac mortality and morbidity, for which no effective treatment exists. Both inflammation and epigenetic regulation play a pivotal role in the early stage of MI/R. The present study aimed at investigating the prospective anti-inflammatory role of trans-anethole (TNA) in targeting MI/R and its related mechanism in upregulating the expression of the inflammatory and cardiac-related gene (VAV3), and its epigenetic regulators (lncRNA-JRKL-AS1 and miR-1298) that were retrieved from in-silico data analysis in an ischemia/reperfusion (I/R) rat model.

Materials & methods: TNA was administered in 3 doses (50, 100, and 200 mg/kg), 15 min prior to coronary ligation in male Wistar rats. The left ventricular end-diastolic pressure and dP/dtmax were assessed. Histopathological, biochemical, and molecular analyses were performed to assess the effects of TNA pre-treatment on the I/R rats model.

Results: TNA alleviated the I/R-induced cardiac injury pathologically and improved the cardiac function tests and enzymes. At the molecular level, TNA upregulated the expression level of the retrieved RNA-based panel (VAV3 mRNA/miR-1298/lncRNA JRKL-AS1). At the protein level, TNA decreased the cardiac content of the pro-inflammatory cytokine TNF-α.

Conclusion: TNA has demonstrated a potential ability to alleviate the cardiac injury and attenuate the inflammatory response following ischemia-reperfusion in the rat model through modulation of the expression of RNA panel (VAV3 mRNA/miR-1298/lncRNA JRKL-AS1) and TNF- α protein.

Keywords: Heart; Ischemia-reperfusion; Rat; Trans-anethole; VAV3 mRNA, miRNA, lncRNA.

MeSH terms

Allylbenzene Derivatives
Animals
Anisoles
Apoptosis
Disease Models, Animal
Epigenesis, Genetic
Male
MicroRNAs* / metabolism
Myocardial Reperfusion Injury* / metabolism
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism
RNA, Messenger / therapeutic use
Rats
Rats, Wistar

Substances

Allylbenzene Derivatives
Anisoles
MicroRNAs
RNA, Long Noncoding
RNA, Messenger
anethole