Relationship between Superoxide Anion Radical and Anion Transport Protein of Red Blood Cell Membrane

Nai-Yang Fu; Er-Xian Zhang; Zhe-Fu Lin; Li-Jun Yu

Relationship between Superoxide Anion Radical and Anion Transport Protein of Red Blood Cell Membrane

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999;31(3):328-330.

Authors

Nai-Yang Fu¹, Er-Xian Zhang, Zhe-Fu Lin, Li-Jun Yu

Affiliation

¹ Department of Biology, Shantou University, Shantou 515063, China. exzhang@mailserv.stu.edu.cn

PMID: 12136189

Abstract

By using a specific inhibitor of anion transport, DIDS, the dependence on band 3 anion transport protein of superoxide anion radical (O(-)(2).) flux across red blood cell (RBC) membrane into RBC was studied and the effect of superoxide anion radical on anion transport in RBC membrane was also discussed based on the hemolytic t(1/2) in an isotonic solution of NH(4)Cl. The results showed that 40 &mgr;mol/L DIDS could completely inhibit anion transport by band 3 protein in RBC membrane, but had no effect on flux into RBC, proving that the O(-)(2). transport into RBC across RBC membrane was independent of band 3 protein the interaction of O(-)(2). with specific SH-group of membrane protein lowered the anion permeability, and it could be reversed by DTT and mercaptoethanol.