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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1984 1
1986 2
1987 1
1988 1
1989 3
1990 10
1991 4
1992 15
1993 5
1994 8
1995 4
1996 10
1997 14
1998 20
1999 20
2000 21
2001 36
2002 46
2003 83
2004 85
2005 139
2006 157
2007 193
2008 234
2009 303
2010 347
2011 439
2012 565
2013 740
2014 935
2015 1141
2016 1273
2017 1497
2018 1942
2019 2390
2020 3006
2021 3708
2022 4995
2023 5143
2024 2772

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28,985 results

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Page 1
Fanzor is a eukaryotic programmable RNA-guided endonuclease.
Saito M, Xu P, Faure G, Maguire S, Kannan S, Altae-Tran H, Vo S, Desimone A, Macrae RK, Zhang F. Saito M, et al. Nature. 2023 Aug;620(7974):660-668. doi: 10.1038/s41586-023-06356-2. Epub 2023 Jun 28. Nature. 2023. PMID: 37380027 Free PMC article.
RNA-guided systems, which use complementarity between a guide RNA and target nucleic acid sequences for recognition of genetic elements, have a central role in biological processes in both prokaryotes and eukaryotes. For example, the prokaryotic CRISPR-Cas systems p …
RNA-guided systems, which use complementarity between a guide RNA and target nucleic acid sequences for recognition of genetic …
Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Zetsche B, et al. Cell. 2015 Oct 22;163(3):759-71. doi: 10.1016/j.cell.2015.09.038. Epub 2015 Sep 25. Cell. 2015. PMID: 26422227 Free PMC article.
The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi-protein complexes, whereas class 2 effectors rely on single-component effector proteins such …
The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nucle …
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector.
Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F. Abudayyeh OO, et al. Science. 2016 Aug 5;353(6299):aaf5573. doi: 10.1126/science.aaf5573. Epub 2016 Jun 2. Science. 2016. PMID: 27256883 Free PMC article.
We characterize the class 2 type VI CRISPR-Cas effector C2c2 and demonstrate its RNA-guided ribonuclease function. C2c2 from the bacterium Leptotrichia shahii provides interference against RNA phage. In vitro biochemical analysis shows that C2c2 is guided by a singl …
We characterize the class 2 type VI CRISPR-Cas effector C2c2 and demonstrate its RNA-guided ribonuclease function. C2c2 from the bact …
RNA-activated protein cleavage with a CRISPR-associated endopeptidase.
Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F. Strecker J, et al. Science. 2022 Nov 25;378(6622):874-881. doi: 10.1126/science.add7450. Epub 2022 Nov 3. Science. 2022. PMID: 36423276 Free PMC article.
In prokaryotes, CRISPR-Cas systems provide adaptive immune responses against foreign genetic elements through RNA-guided nuclease activity. Recently, additional genes with non-nuclease functions have been found in genetic association with CRISPR systems, suggesting that th …
In prokaryotes, CRISPR-Cas systems provide adaptive immune responses against foreign genetic elements through RNA-guided nuclease act …
Rationally engineered Cas9 nucleases with improved specificity.
Slaymaker IM, Gao L, Zetsche B, Scott DA, Yan WX, Zhang F. Slaymaker IM, et al. Science. 2016 Jan 1;351(6268):84-8. doi: 10.1126/science.aad5227. Epub 2015 Dec 1. Science. 2016. PMID: 26628643 Free PMC article.
Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-target sites that are not fully complementary to the guide, which poses a major challenge for genome editing. Here, we use structure- …
Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-ta …
Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d.
Zhang C, Konermann S, Brideau NJ, Lotfy P, Wu X, Novick SJ, Strutzenberg T, Griffin PR, Hsu PD, Lyumkis D. Zhang C, et al. Cell. 2018 Sep 20;175(1):212-223.e17. doi: 10.1016/j.cell.2018.09.001. Cell. 2018. PMID: 30241607 Free PMC article.
Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and their signature Cas13d ribonuclease employs guide RNAs to cleave matching target RNAs. To understand the molecular basis for Cas13d function and explain its compact molecular arc …
Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and their signature Cas13d ribonuclease employs …
Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity.
Tsuchida CA, Zhang S, Doost MS, Zhao Y, Wang J, O'Brien E, Fang H, Li CP, Li D, Hai ZY, Chuck J, Brötzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA, Liu JG. Tsuchida CA, et al. Mol Cell. 2022 Mar 17;82(6):1199-1209.e6. doi: 10.1016/j.molcel.2022.02.002. Epub 2022 Feb 25. Mol Cell. 2022. PMID: 35219382 Free PMC article.
A compact protein with a size of <1,000 amino acids, the CRISPR-associated protein CasX is a fundamentally distinct RNA-guided nuclease when compared to Cas9 and Cas12a. Although it can induce RNA-guided genome editing in mammalian cells, the activity of CasX is …
A compact protein with a size of <1,000 amino acids, the CRISPR-associated protein CasX is a fundamentally distinct RNA-guided nuc …
Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA.
Yamano T, Nishimasu H, Zetsche B, Hirano H, Slaymaker IM, Li Y, Fedorova I, Nakane T, Makarova KS, Koonin EV, Ishitani R, Zhang F, Nureki O. Yamano T, et al. Cell. 2016 May 5;165(4):949-62. doi: 10.1016/j.cell.2016.04.003. Epub 2016 Apr 21. Cell. 2016. PMID: 27114038 Free PMC article.
Cpf1 is an RNA-guided endonuclease of a type V CRISPR-Cas system that has been recently harnessed for genome editing. Here, we report the crystal structure of Acidaminococcus sp. Cpf1 (AsCpf1) in complex with the guide RNA and its target DNA at 2.8 A resolution. AsC …
Cpf1 is an RNA-guided endonuclease of a type V CRISPR-Cas system that has been recently harnessed for genome editing. Here, we report …
The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a.
Liu L, Li X, Ma J, Li Z, You L, Wang J, Wang M, Zhang X, Wang Y. Liu L, et al. Cell. 2017 Aug 10;170(4):714-726.e10. doi: 10.1016/j.cell.2017.06.050. Epub 2017 Jul 27. Cell. 2017. PMID: 28757251 Free article.
Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential applications in RNA technology. ...The crRNA-target RNA duplex binds in a positively charged central channel of the nuclease (NUC) lobe, and …
Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential a …
Guiding flying-spot laser transepithelial phototherapeutic keratectomy with optical coherence tomography.
Li Y, Yokogawa H, Tang M, Chamberlain W, Zhang X, Huang D. Li Y, et al. J Cataract Refract Surg. 2017 Apr;43(4):525-536. doi: 10.1016/j.jcrs.2017.03.004. J Cataract Refract Surg. 2017. PMID: 28532939 Free PMC article. Review.
PURPOSE: To analyze transepithelial phototherapeutic keratectomy (PTK) results using optical coherence tomography (OCT) and develop a model to guide the laser dioptric and depth settings. SETTING: Casey Eye Institute, Portland, Oregon, USA. ...CONCLUSIONS: The OCT-measured …
PURPOSE: To analyze transepithelial phototherapeutic keratectomy (PTK) results using optical coherence tomography (OCT) and develop a model …
28,985 results
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