Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My NCBI Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
2003 1
2009 1
2010 2
2011 1
2012 1
2013 2
2024 0

Text availability

Article attribute

Article type

Publication date

Search Results

8 results

Results by year

Filters applied: . Clear all
Page 1
Did you mean eugene yu (151 results)?
Construction of the ie1-Bacmid expression system and its use to express EGFP and BmAGO2 in BmN cells.
Zhou F, Gao Z, Lv Z, Chen J, Hong Y, Yu W, Wang D, Jiang C, Wu X, Zhang Y, Nie Z. Zhou F, et al. Appl Biochem Biotechnol. 2013 Apr;169(8):2237-47. doi: 10.1007/s12010-013-0137-y. Epub 2013 Feb 24. Appl Biochem Biotechnol. 2013. PMID: 23436226
In this work, we constructed and screened a eukaryotic expression vector for silkworm cells The EGFP and B. mori Argonaute2 proteins were found to be efficiently expressed using the screened pIEx-1 vector with the FuGENE 6 transfection reagent. Additionally, we constructed …
In this work, we constructed and screened a eukaryotic expression vector for silkworm cells The EGFP and B. mori Argonaute2 proteins were fo …
Helicobacter pylori CagA inhibits the expression of Runx3 via Src/MEK/ERK and p38 MAPK pathways in gastric epithelial cell.
Liu Z, Xu X, Chen L, Li W, Sun Y, Zeng J, Yu H, Chen C, Jia J. Liu Z, et al. J Cell Biochem. 2012 Mar;113(3):1080-6. doi: 10.1002/jcb.23440. J Cell Biochem. 2012. PMID: 22266963
Human gastric epithelial immortalized GES-1 cells were transfected with CagA-expression vector or control vector with FuGENE HD transfection reagent. Runx3 expression levels were determined by QRT-PCR and immunoblotting. ...
Human gastric epithelial immortalized GES-1 cells were transfected with CagA-expression vector or control vector with FuGENE HD trans …
Interaction of 14-3-3σ with KCMF1 suppresses the proliferation and colony formation of human colon cancer stem cells.
Zou J, Mi L, Yu XF, Dong J. Zou J, et al. World J Gastroenterol. 2013 Jun 28;19(24):3770-80. doi: 10.3748/wjg.v19.i24.3770. World J Gastroenterol. 2013. PMID: 23840115 Free PMC article.
For the subsequent coimmunoprecipitation assay, the plasmids PCDEF-Flag-14-3-3sigma, PCDEF-Myc-KCMF1, PCDEF-Myc-NQO2 and PCDEF-Myc-HIBADH were successfully constructed, and the sequences were further confirmed by DNA sequencing. The Fugene 6 reagent was used to transfect t …
For the subsequent coimmunoprecipitation assay, the plasmids PCDEF-Flag-14-3-3sigma, PCDEF-Myc-KCMF1, PCDEF-Myc-NQO2 and PCDEF-Myc-HIBADH we …
[Long-term and stable expression of transgene mediated by piggyBac transposon in gynecological malignant tumor cells].
Kang Y, Sun QW, Yu WB, Cheng MJ, Zhang XY, Wu X, Chen CM, Zheng YF, Xu CJ. Kang Y, et al. Zhonghua Fu Chan Ke Za Zhi. 2010 Apr;45(4):292-7. Zhonghua Fu Chan Ke Za Zhi. 2010. PMID: 20646543 Chinese.
METHODS: Amplified herpes simplex virus thymidine kinase (HSV-tk) gene coding region by PCR and integrated it into PB expression vector, PB[Act-RFP]DS, for reconstructing PB[Act-RFP, HSV-tk]DS (pPB/TK). By using different transfection reagents: FuGENE HD, jetPEI, lipofecta …
METHODS: Amplified herpes simplex virus thymidine kinase (HSV-tk) gene coding region by PCR and integrated it into PB expression vector, PB[ …
[Construction of FLT3 3'-UTR-luciferase reporter vector and evaluation of its activity].
Gao XN, Lin J, Li YH, Wang LL, Yu L. Gao XN, et al. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Jun;18(3):694-7. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010. PMID: 20561431 Chinese.
The luciferase reporter vector and miRNA eukaryotic expression vector were transferred into 293T cells using FuGENE HD transfection reagent. The Dual-Luciferase Reporter Assay System was used to quantitate the reporter activity. ...
The luciferase reporter vector and miRNA eukaryotic expression vector were transferred into 293T cells using FuGENE HD transfection r …
[Construction of eukaryotic expression vector encoding ACRBP and its expression in hepatocarcinoma cells].
Luo B, Yun X, Lin YD, Xiao SW, Yan GH, He SJ, He SJ, Chen F, Xie XX. Luo B, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Oct;27(10):1072-4. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011. PMID: 21968305 Chinese.
The PCR product was ligated into an eukaryotic expression vector pEGFP-N1 to construct a recombinant plasmid pEGFP-N1/ACRBP. Then the pEGFP-N1/ACRBP was transfected by Fugene HD into ACRBP-negative HepG2 cells. The stably transfected clones were selected by G418. ...
The PCR product was ligated into an eukaryotic expression vector pEGFP-N1 to construct a recombinant plasmid pEGFP-N1/ACRBP. Then the pEGFP- …
[Construction of the eukaryotic expression vector of mouse beta-NGF and its expression in NIH 3T3 fibroblast cell lines].
Song M, Chen SZ. Song M, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2003 Mar;19(2):124-6. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2003. PMID: 15151745 Chinese.
NIH 3T3 cell grown to log phase was transfected with this vector using FuGENE(TM) 6 transfection reagent. The transfected cells were grown in DMEM medium containing G418 at 72 hours after transfection, and the positive clones were selected in G418 medium until the 20 th da …
NIH 3T3 cell grown to log phase was transfected with this vector using FuGENE(TM) 6 transfection reagent. The transfected cells were …
[Construction of pSG5/NS5A5BdeltaC and its expression in Huh7 cells].
Wang XP, Li FJ, Deng L, Nagano-Fujii M, Kitayama K, Hotta H. Wang XP, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009 Jan;25(1):31-4. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009. PMID: 20104685 Chinese.
The positive clones were screened by Ampicillin and identified by the restriction endoenzyme Sac I and Bgl II digestion and agarose gel electrophoresis. The constructs were transfected into Huh7 cells with FuGene 6 reagents. Immunofluorescence and Western blot were perform …
The positive clones were screened by Ampicillin and identified by the restriction endoenzyme Sac I and Bgl II digestion and agarose gel elec …