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Anti-C1q Autoantibodies: Standard Quantification and Innovative ELISA.
Csorba K, Schirmbeck L, Dubler D, Trendelenburg M. Csorba K, et al. Methods Mol Biol. 2021;2227:107-114. doi: 10.1007/978-1-0716-1016-9_10. Methods Mol Biol. 2021. PMID: 33847935
More recently, a linear epitope on the C1q A chain, that is targeted by anti-C1q, has been described (A08). The assay using this peptide seems to be more specific and more sensitive for the detection of active nephritis in SLE patients than the conventional anti-C1q ass …
More recently, a linear epitope on the C1q A chain, that is targeted by anti-C1q, has been described (A08). The assay using this pept …
Autoantibodies Against Albumin in Patients With Systemic Lupus Erythematosus.
Nehring J, Schirmbeck LA, Friebus-Kardash J, Dubler D, Huynh-Do U, Chizzolini C, Ribi C, Trendelenburg M. Nehring J, et al. Front Immunol. 2018 Oct 2;9:2090. doi: 10.3389/fimmu.2018.02090. eCollection 2018. Front Immunol. 2018. PMID: 30333817 Free PMC article. Clinical Trial.
Methods: Sera of 180 SLE patients included to the Swiss SLE Cohort Study and 188 age- and sex-matched healthy controls were evaluated. Levels of anti-HSA IgG and anti-BSA IgG were quantified by ELISA. Selected samples were further characterized using serum fractions obtain …
Methods: Sera of 180 SLE patients included to the Swiss SLE Cohort Study and 188 age- and sex-matched healthy controls were evaluated. Level …
IL-12/IL-18-dependent IFN-gamma release by murine dendritic cells.
Stober D, Schirmbeck R, Reimann J. Stober D, et al. J Immunol. 2001 Jul 15;167(2):957-65. doi: 10.4049/jimmunol.167.2.957. J Immunol. 2001. PMID: 11441104
Stimulation of in vitro-generated DC with exogenous IL-12 and IL-18 (but not with IL-4 or LPS plus IL-18) induced IFN-gamma expression and release in 15-20% of the DC (detectable by FACS analyses or ELISA). Endogenous IL-12 p70 produced by DC in response to ligation of CD4 …
Stimulation of in vitro-generated DC with exogenous IL-12 and IL-18 (but not with IL-4 or LPS plus IL-18) induced IFN-gamma expression and r …
Efficient vaccination by intradermal or intramuscular inoculation of plasmid DNA expressing hepatitis B surface antigen under desmin promoter/enhancer control.
Kwissa M, von Kampen vK, Zurbriggen R, Glück R, Reimann J, Schirmbeck R. Kwissa M, et al. Vaccine. 2000 May 8;18(22):2337-44. doi: 10.1016/s0264-410x(00)00030-x. Vaccine. 2000. PMID: 10738089
Cells of different species and tissue origin transiently transfected in vitro with pCI/S or pDes/S plasmid DNA expressed readily detectable amounts of HBsAg, either intracellularly (precipitated from cell lysates), or as secreted products (detectable in ELISA). When these …
Cells of different species and tissue origin transiently transfected in vitro with pCI/S or pDes/S plasmid DNA expressed readily detectable …
Characterization of complex B cell epitopes on woodchuck hepatitis virus surface antigens by using plasmids encoding chimeric proteins and DNA immunization.
Zheng X, Schirmbeck R, Hilken G, Waters JA, Yang D, Reimann J, Roggendorf M, Lu M. Zheng X, et al. Virology. 2002 Mar 15;294(2):342-53. doi: 10.1006/viro.2001.1338. Virology. 2002. PMID: 12009876 Free article.
MCSAgs and CSAgs were expressed in transiently transfected mammalian cells and were reactive to anti-HBsAg and anti-WHsAg, as shown by indirect immunofluorescence staining and ELISA. Vaccination with plasmids encoding MCSAgs induced strong antibody responses to the pre-S2 …
MCSAgs and CSAgs were expressed in transiently transfected mammalian cells and were reactive to anti-HBsAg and anti-WHsAg, as shown by indir …