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Page 1
Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive.
Li F, Ye L, Zhang L, Li X, Liu X, Zhu J, Li H, Pang H, Yan Y, Xu L, Yang M, Yan J. Li F, et al. Mater Today Bio. 2022 Apr 8;14:100256. doi: 10.1016/j.mtbio.2022.100256. eCollection 2022 Mar. Mater Today Bio. 2022. PMID: 35469253 Free PMC article.
Here, we report a genetically programmed self-assembly living-cell bioadhesive inspired by barnacle and curli system. Firstly, the encoding genes of two natural adhesion proteins (CsgA and cp19k) derived from E. coli curli and barnacle cement were fused and expresse …
Here, we report a genetically programmed self-assembly living-cell bioadhesive inspired by barnacle and curli system. Firstly, the en …
Engineered cytochrome fused extracellular matrix enabled efficient extracellular electron transfer and improved performance of microbial fuel cell.
Chen YY, Yang FQ, Xu N, Wang XQ, Xie PC, Wang YZ, Fang Z, Yong YC. Chen YY, et al. Sci Total Environ. 2022 Jul 15;830:154806. doi: 10.1016/j.scitotenv.2022.154806. Epub 2022 Mar 25. Sci Total Environ. 2022. PMID: 35341857
Here, a new strategy to reinforce the EET by engineering synthetic extracellular matrix (ECM) with cytochrome fused curli was developed. By genetically fusing a minimal cytochrome domain (MCD) with the curli protein CsgA and heterogeneously expressing in model exoel …
Here, a new strategy to reinforce the EET by engineering synthetic extracellular matrix (ECM) with cytochrome fused curli was develop …
Synthesis of robust underwater glues from common proteins via unfolding-aggregating strategy.
Liu Y, Li K, Tian J, Gao A, Tian L, Su H, Miao S, Tao F, Ren H, Yang Q, Cao J, Yang P. Liu Y, et al. Nat Commun. 2023 Aug 24;14(1):5145. doi: 10.1038/s41467-023-40856-z. Nat Commun. 2023. PMID: 37620335 Free PMC article.
However, except for specific proteins such as mussel adhesive protein, barnacle cement proteins, curli protein and their related recombinant proteins, it is believed that abundant common proteins cannot be converted into underwater glue. ...
However, except for specific proteins such as mussel adhesive protein, barnacle cement proteins, curli protein and their related reco …
Dictamnine Inhibits the Adhesion to and Invasion of Uropathogenic Escherichia Coli (UPEC) to Urothelial Cells.
Yang W, Liu P, Chen Y, Lv Q, Wang Z, Huang W, Jiang H, Zheng Y, Jiang Y, Sun L. Yang W, et al. Molecules. 2022 Jan 2;27(1):272. doi: 10.3390/molecules27010272. Molecules. 2022. PMID: 35011504 Free PMC article.
RT-qPCR analysis showed that treatment downregulated the expression of type 1 fimbriae, P fimbriae, and curli fimbriae adhesion genes, and also downregulated adhesion-related receptor genes of uroepithelial cells. ...
RT-qPCR analysis showed that treatment downregulated the expression of type 1 fimbriae, P fimbriae, and curli fimbriae adhesion genes …
Comparative Genomic Analyses of Escherichia coli from a Meat Processing Environment in Relation to Their Biofilm Formation and Persistence.
Yang X, Tran F, Zhang P. Yang X, et al. Microbiol Spectr. 2023 Jun 15;11(3):e0018323. doi: 10.1128/spectrum.00183-23. Epub 2023 May 15. Microbiol Spectr. 2023. PMID: 37184412 Free PMC article.

A retrospective analysis of phenotypes found a significant correlation (P < 0.05) between BF ability and curli production, cellulose synthesis, and/or mobility. However, the BF group also included strains that were negative for curli and cellulose and/or missing

…

A retrospective analysis of phenotypes found a significant correlation (P < 0.05) between BF ability and curli production, cellulo

…
Socializing at the Air-Liquid Interface: a Functional Genomic Analysis on Biofilm-Related Genes during Pellicle Formation by Escherichia coli and Its Interaction with Aeromonas australiensis.
Xu ZS, Zhu T, Wang Z, Yang X, Gänzle MG. Xu ZS, et al. Appl Environ Microbiol. 2023 Jul 26;89(7):e0045623. doi: 10.1128/aem.00456-23. Epub 2023 Jun 13. Appl Environ Microbiol. 2023. PMID: 37310210 Free PMC article.
Here, we report that pellicle-forming strains do not harbor unique genes relative to non-pellicle-forming strains; however, the expression level of biofilm-related genes differed, especially for the genes encoding curli. Further, the regulatory region of curli biosy …
Here, we report that pellicle-forming strains do not harbor unique genes relative to non-pellicle-forming strains; however, the expression l …
Biofilm formation by Non-O157 Shiga toxin-producing Escherichia coli in monocultures and co-cultures with meat processing surface bacteria.
Fang Y, Visvalingam J, Zhang P, Yang X. Fang Y, et al. Food Microbiol. 2022 Apr;102:103902. doi: 10.1016/j.fm.2021.103902. Epub 2021 Sep 14. Food Microbiol. 2022. PMID: 34809934
Biofilm development by 50 MPB and 6 STEC strains in mono- and co-cultures was assessed by the crystal violet staining method, and their expression of curli and cellulose was determined using the Congo red agar method. ...The O45 strain, despite having the greatest number o …
Biofilm development by 50 MPB and 6 STEC strains in mono- and co-cultures was assessed by the crystal violet staining method, and their expr …
Characterization of uropathogenic Escherichia coli phylogroups associated with antimicrobial resistance, virulence factor distribution, and virulence-related phenotypes.
Wang MC, Fan YH, Zhang YZ, Bregente CJB, Lin WH, Chen CA, Lin TP, Kao CY. Wang MC, et al. Infect Genet Evol. 2023 Oct;114:105493. doi: 10.1016/j.meegid.2023.105493. Epub 2023 Aug 25. Infect Genet Evol. 2023. PMID: 37634856 Free article.
Compared to phylogroup B2 E. coli, phylogroups F and clade I E. coli had higher motility while phylogroup C E. coli had lower motility. >60% of phylogroups A and C E. coli showed very low curli production. In contrast, 14%, 10%, and 7%, of E. coli in phylogroups F, B2, …
Compared to phylogroup B2 E. coli, phylogroups F and clade I E. coli had higher motility while phylogroup C E. coli had lower motility. > …
Engineering a novel c-di-GMP-binding protein for biofilm dispersal.
Ma Q, Yang Z, Pu M, Peti W, Wood TK. Ma Q, et al. Environ Microbiol. 2011 Mar;13(3):631-42. doi: 10.1111/j.1462-2920.2010.02368.x. Epub 2010 Nov 8. Environ Microbiol. 2011. PMID: 21059164 Free PMC article.
Since BdcA increases motility and extracellular DNA production while decreasing exopolysaccharide, cell length and aggregation, we reasoned that BdcA decreases the concentration of c-di-GMP, the intracellular messenger that controls cell motility through flagellar rotation and bi …
Since BdcA increases motility and extracellular DNA production while decreasing exopolysaccharide, cell length and aggregation, we reasoned …
Biofilm-Forming Capacity of Escherichia coli Isolated from Cattle and Beef Packing Plants: Relation to Virulence Attributes, Stage of Processing, Antimicrobial Interventions, and Heat Tolerance.
Stanford K, Tran F, Zhang P, Yang X. Stanford K, et al. Appl Environ Microbiol. 2021 Nov 10;87(23):e0112621. doi: 10.1128/AEM.01126-21. Epub 2021 Sep 22. Appl Environ Microbiol. 2021. PMID: 34550756 Free PMC article.
To identify selection pressures for biofilm, we evaluated 745 isolates from cattle and 700 generic E. coli isolates from two beef slaughter plants for motility, the expression of curli and cellulose, and biofilm-forming potential. Cattle isolates were also screened for ser …
To identify selection pressures for biofilm, we evaluated 745 isolates from cattle and 700 generic E. coli isolates from two beef slaughter …
18 results