Construction of a novel filamentous fungal protein expression system based on redesigning of regulatory elements.
Zhang Z, Xiang B, Zhao S, Yang L, Chen Y, Hu Y, Hu S.
Zhang Z, et al.
Appl Microbiol Biotechnol. 2022 Jan;106(2):647-661. doi: 10.1007/s00253-022-11761-0. Epub 2022 Jan 12.
Appl Microbiol Biotechnol. 2022.
PMID: 35019997
It was found that the proteins exhibited high purity in the culture supernatant after cultivation with starch. Two inducible promoters, Pamy15A and PempA, under the activation of the transcription factor AmyR were used as elements in the construction of versatile vectors. …
It was found that the proteins exhibited high purity in the culture supernatant after cultivation with starch. Two inducible promoters, Pamy …