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Bivalent intra-spike binding provides durability against emergent Omicron lineages: Results from a global consortium.
Callaway HM, Hastie KM, Schendel SL, Li H, Yu X, Shek J, Buck T, Hui S, Bedinger D, Troup C, Dennison SM, Li K, Alpert MD, Bailey CC, Benzeno S, Bonnevier JL, Chen JQ, Chen C, Cho H, Crompton PD, Dussupt V, Entzminger KC, Ezzyat Y, Fleming JK, Geukens N, Gilbert AE, Guan Y, Han X, Harvey CJ, Hatler JM, Howie B, Hu C, Huang A, Imbrechts M, Jin A, Kamachi N, Keitany G, Klinger M, Kolls JK, Krebs SJ, Li T, Luo F, Maruyama T, Meehl MA, Mendez-Rivera L, Musa A, Okumura CJ, Rubin BER, Sato AK, Shen M, Singh A, Song S, Tan J, Trimarchi JM, Upadhyay DP, Wang Y, Yu L, Yuan TZ, Yusko E, Peters B, Tomaras G, Saphire EO. Callaway HM, et al. Among authors: entzminger kc. Cell Rep. 2023 Jan 31;42(1):112014. doi: 10.1016/j.celrep.2023.112014. Epub 2023 Jan 12. Cell Rep. 2023. PMID: 36681898 Free PMC article.
Increased Fab thermoresistance via VH-targeted directed evolution.
Entzminger KC, Johnson JL, Hyun J, Lieberman RL, Maynard JA. Entzminger KC, et al. Protein Eng Des Sel. 2015 Oct;28(10):365-77. doi: 10.1093/protein/gzv037. Epub 2015 Aug 16. Protein Eng Des Sel. 2015. PMID: 26283664 Free PMC article.
Structural and biophysical characterization of an epitope-specific engineered Fab fragment and complexation with membrane proteins: implications for co-crystallization.
Johnson JL, Entzminger KC, Hyun J, Kalyoncu S, Heaner DP Jr, Morales IA, Sheppard A, Gumbart JC, Maynard JA, Lieberman RL. Johnson JL, et al. Among authors: entzminger kc. Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):896-906. doi: 10.1107/S1399004715001856. Epub 2015 Mar 27. Acta Crystallogr D Biol Crystallogr. 2015. PMID: 25849400 Free PMC article.
Crystallization chaperone strategies for membrane proteins.
Lieberman RL, Culver JA, Entzminger KC, Pai JC, Maynard JA. Lieberman RL, et al. Among authors: entzminger kc. Methods. 2011 Dec;55(4):293-302. doi: 10.1016/j.ymeth.2011.08.004. Epub 2011 Aug 11. Methods. 2011. PMID: 21854852 Free PMC article. Review.