Kojima K - Search Results

1

Recombinase polymerase amplification using novel thermostable strand-displacing DNA polymerases from Aeribacillus pallidus and Geobacillus zalihae.

Juma KM, Inoue E, Asada K, Fukuda W, Morimoto K, Yamagata M, Takita T, Kojima K, Suzuki K, Nakura Y, Yanagihara I, Fujiwara S, Yasukawa K. Juma KM, et al. Among authors: kojima k. J Biosci Bioeng. 2023 Apr;135(4):282-290. doi: 10.1016/j.jbiosc.2023.01.009. Epub 2023 Feb 17. J Biosci Bioeng. 2023. PMID: 36806411

2

Preparation and characterization of the RNase H domain of Moloney murine leukemia virus reverse transcriptase.

Nishimura K, Yokokawa K, Hisayoshi T, Fukatsu K, Kuze I, Konishi A, Mikami B, Kojima K, Yasukawa K. Nishimura K, et al. Among authors: kojima k. Protein Expr Purif. 2015 Sep;113:44-50. doi: 10.1016/j.pep.2015.04.012. Epub 2015 May 7. Protein Expr Purif. 2015. PMID: 25959458 Free article.

3

Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase.

Okano H, Katano Y, Baba M, Fujiwara A, Hidese R, Fujiwara S, Yanagihara I, Hayashi T, Kojima K, Takita T, Yasukawa K. Okano H, et al. Among authors: kojima k. Enzyme Microb Technol. 2017 Jan;96:111-120. doi: 10.1016/j.enzmictec.2016.10.003. Epub 2016 Oct 11. Enzyme Microb Technol. 2017. PMID: 27871370

4

The cleavage site preference of the porcine pepsin on the N-terminal α1 chain of bovine type I collagen: a focal analysis with mass spectrometry.

Qian J, Ito S, Satoh J, Geng H, Tanaka K, Hattori S, Kojima K, Takita T, Yasukawa K. Qian J, et al. Among authors: kojima k. Biosci Biotechnol Biochem. 2017 Mar;81(3):514-522. doi: 10.1080/09168451.2016.1263146. Epub 2016 Dec 9. Biosci Biotechnol Biochem. 2017. PMID: 27931164

5

High sensitive one-step RT-PCR using MMLV reverse transcriptase, DNA polymerase with reverse transcriptase activity, and DNA/RNA helicase.

Okano H, Baba M, Yamasaki T, Hidese R, Fujiwara S, Yanagihara I, Ujiiye T, Hayashi T, Kojima K, Takita T, Yasukawa K. Okano H, et al. Among authors: kojima k. Biochem Biophys Res Commun. 2017 May 20;487(1):128-133. doi: 10.1016/j.bbrc.2017.04.030. Epub 2017 Apr 8. Biochem Biophys Res Commun. 2017. PMID: 28400277 No abstract available.

6

Effects of neutral salts and pH on the activity and stability of human RNase H2.

Baba M, Kojima K, Nakase R, Imai S, Yamasaki T, Takita T, Crouch RJ, Yasukawa K. Baba M, et al. Among authors: kojima k. J Biochem. 2017 Sep 1;162(3):211-219. doi: 10.1093/jb/mvx021. J Biochem. 2017. PMID: 28402412 Free PMC article.

7

Next-generation sequencing-based analysis of reverse transcriptase fidelity.

Yasukawa K, Iida K, Okano H, Hidese R, Baba M, Yanagihara I, Kojima K, Takita T, Fujiwara S. Yasukawa K, et al. Among authors: kojima k. Biochem Biophys Res Commun. 2017 Oct 14;492(2):147-153. doi: 10.1016/j.bbrc.2017.07.169. Epub 2017 Aug 1. Biochem Biophys Res Commun. 2017. PMID: 28778390

8

Further increase in thermostability of Moloney murine leukemia virus reverse transcriptase by mutational combination.

Baba M, Kakue R, Leucht C, Rasor P, Walch H, Ladiges D, Bell C, Kojima K, Takita T, Yasukawa K. Baba M, et al. Among authors: kojima k. Protein Eng Des Sel. 2017 Aug 1;30(8):551-557. doi: 10.1093/protein/gzx046. Protein Eng Des Sel. 2017. PMID: 28967961

9

High sensitive RNA detection by one-step RT-PCR using the genetically engineered variant of DNA polymerase with reverse transcriptase activity from hyperthermophilies.

Okano H, Baba M, Kawato K, Hidese R, Yanagihara I, Kojima K, Takita T, Fujiwara S, Yasukawa K. Okano H, et al. Among authors: kojima k. J Biosci Bioeng. 2018 Mar;125(3):275-281. doi: 10.1016/j.jbiosc.2017.10.004. J Biosci Bioeng. 2018. PMID: 29100684

10

Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system.

Katano Y, Li T, Baba M, Nakamura M, Ito M, Kojima K, Takita T, Yasukawa K. Katano Y, et al. Among authors: kojima k. Biosci Biotechnol Biochem. 2017 Dec;81(12):2339-2345. doi: 10.1080/09168451.2017.1394790. Epub 2017 Nov 6. Biosci Biotechnol Biochem. 2017. PMID: 29103348

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