Optimizing an artificial metabolic pathway: engineering the cofactor specificity of Corynebacterium 2,5-diketo-D-gluconic acid reductase for use in vitamin C biosynthesis.
Banta S, Swanson BA, Wu S, Jarnagin A, Anderson S.
Banta S, et al.
Biochemistry. 2002 May 21;41(20):6226-36. doi: 10.1021/bi015987b.
Biochemistry. 2002.
PMID: 12009883
These mutations conferred greater activity with NADH upon the Corynebacterium 2,5-DKG reductase [Banta, S., Swanson, B. A., Wu, S., Jarnagin, A., and Anderson, S. (2002) Protein Eng. 15, 131-140; (1)]. ...
These mutations conferred greater activity with NADH upon the Corynebacterium 2,5-DKG reductase [Banta, S., Swanson, B. A., Wu …