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Engineering of complex protein sialylation in plants.
Kallolimath S, Castilho A, Strasser R, Grünwald-Gruber C, Altmann F, Strubl S, Galuska CE, Zlatina K, Galuska SP, Werner S, Thiesler H, Werneburg S, Hildebrandt H, Gerardy-Schahn R, Steinkellner H. Kallolimath S, et al. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):9498-503. doi: 10.1073/pnas.1604371113. Epub 2016 Jul 21. Proc Natl Acad Sci U S A. 2016. PMID: 27444013 Free PMC article.
An oligosaccharyltransferase from Leishmania major increases the N-glycan occupancy on recombinant glycoproteins produced in Nicotiana benthamiana.
Castilho A, Beihammer G, Pfeiffer C, Göritzer K, Montero-Morales L, Vavra U, Maresch D, Grünwald-Gruber C, Altmann F, Steinkellner H, Strasser R. Castilho A, et al. Plant Biotechnol J. 2018 Oct;16(10):1700-1709. doi: 10.1111/pbi.12906. Epub 2018 Mar 25. Plant Biotechnol J. 2018. PMID: 29479800 Free PMC article.
Generation of enzymatically competent SARS-CoV-2 decoy receptor ACE2-Fc in glycoengineered Nicotiana benthamiana.
Castilho A, Schwestka J, Kienzl NF, Vavra U, Grünwald-Gruber C, Izadi S, Hiremath C, Niederhöfer J, Laurent E, Monteil V, Mirazimi A, Wirnsberger G, Stadlmann J, Stöger E, Mach L, Strasser R. Castilho A, et al. Biotechnol J. 2021 Jun;16(6):e2000566. doi: 10.1002/biot.202000566. Epub 2021 Feb 12. Biotechnol J. 2021. PMID: 33481336 Free PMC article.
A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests.
Klausberger M, Duerkop M, Haslacher H, Wozniak-Knopp G, Cserjan-Puschmann M, Perkmann T, Lingg N, Aguilar PP, Laurent E, De Vos J, Hofner M, Holzer B, Stadler M, Manhart G, Vierlinger K, Egger M, Milchram L, Gludovacz E, Marx N, Köppl C, Tauer C, Beck J, Maresch D, Grünwald-Gruber C, Strobl F, Satzer P, Stadlmayr G, Vavra U, Huber J, Wahrmann M, Eskandary F, Breyer MK, Sieghart D, Quehenberger P, Leitner G, Strassl R, Egger AE, Irsara C, Griesmacher A, Hoermann G, Weiss G, Bellmann-Weiler R, Loeffler-Ragg J, Borth N, Strasser R, Jungbauer A, Hahn R, Mairhofer J, Hartmann B, Binder NB, Striedner G, Mach L, Weinhäusel A, Dieplinger B, Grebien F, Gerner W, Binder CJ, Grabherr R. Klausberger M, et al. EBioMedicine. 2021 May;67:103348. doi: 10.1016/j.ebiom.2021.103348. Epub 2021 Apr 25. EBioMedicine. 2021. PMID: 33906067 Free PMC article.
N-Glycosylation of the SARS-CoV-2 Receptor Binding Domain Is Important for Functional Expression in Plants.
Shin YJ, König-Beihammer J, Vavra U, Schwestka J, Kienzl NF, Klausberger M, Laurent E, Grünwald-Gruber C, Vierlinger K, Hofner M, Margolin E, Weinhäusel A, Stöger E, Mach L, Strasser R. Shin YJ, et al. Front Plant Sci. 2021 Jun 15;12:689104. doi: 10.3389/fpls.2021.689104. eCollection 2021. Front Plant Sci. 2021. PMID: 34211491 Free PMC article.
Impact of Specific N-Glycan Modifications on the Use of Plant-Produced SARS-CoV-2 Antigens in Serological Assays.
Schwestka J, König-Beihammer J, Shin YJ, Vavra U, Kienzl NF, Grünwald-Gruber C, Maresch D, Klausberger M, Laurent E, Stadler M, Manhart G, Huber J, Hofner M, Vierlinger K, Weinhäusel A, Swoboda I, Binder CJ, Gerner W, Grebien F, Altmann F, Mach L, Stöger E, Strasser R. Schwestka J, et al. Front Plant Sci. 2021 Sep 27;12:747500. doi: 10.3389/fpls.2021.747500. eCollection 2021. Front Plant Sci. 2021. PMID: 34646292 Free PMC article.
Designed SARS-CoV-2 receptor binding domain variants form stable monomers.
Klausberger M, Kienzl NF, Stadlmayr G, Grünwald-Gruber C, Laurent E, Stadlbauer K, Stracke F, Vierlinger K, Hofner M, Manhart G, Gerner W, Grebien F, Weinhäusel A, Mach L, Wozniak-Knopp G. Klausberger M, et al. Biotechnol J. 2022 May;17(5):e2100422. doi: 10.1002/biot.202100422. Epub 2022 Feb 3. Biotechnol J. 2022. PMID: 35078277 Free PMC article.
56 results