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Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering.
Raes L, Pille M, Harizaj A, Goetgeluk G, Van Hoeck J, Stremersch S, Fraire JC, Brans T, de Jong OG, Maas-Bakker R, Mastrobattista E, Vader P, De Smedt SC, Vandekerckhove B, Raemdonck K, Braeckmans K. Raes L, et al. Among authors: brans t. Mol Ther Nucleic Acids. 2021 Aug 19;25:696-707. doi: 10.1016/j.omtn.2021.08.014. eCollection 2021 Sep 3. Mol Ther Nucleic Acids. 2021. PMID: 34589287 Free PMC article.
Vapor nanobubble is the more reliable photothermal mechanism for inducing endosomal escape of siRNA without disturbing cell homeostasis.
Fraire JC, Houthaeve G, Liu J, Raes L, Vermeulen L, Stremersch S, Brans T, García-Díaz Barriga G, De Keulenaer S, Van Nieuwerburgh F, De Rycke R, Vandesompele J, Mestdagh P, Raemdonck K, De Vos WH, De Smedt S, Braeckmans K. Fraire JC, et al. Among authors: brans t. J Control Release. 2020 Mar 10;319:262-275. doi: 10.1016/j.jconrel.2019.12.050. Epub 2020 Jan 2. J Control Release. 2020. PMID: 31904400
Cytosolic delivery of gadolinium via photoporation enables improved in vivo magnetic resonance imaging of cancer cells.
Harizaj A , Descamps B , Mangodt C , Stremersch S , Stoppa A , Balcaen L , Brans T , De Rooster H , Devriendt N , Fraire JC , Bolea-Fernandez E , De Wever O , Willaert W , Vanhaecke F , Stevens CV , De Smedt SC , Roman B , Vanhove C , Lentacker I , Braeckmans K . Harizaj A , et al. Among authors: brans t. Biomater Sci. 2021 Jun 7;9(11):4005-4018. doi: 10.1039/d1bm00479d. Epub 2021 Apr 26. Biomater Sci. 2021. PMID: 33899850
Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation.
Liu J, Li C, Brans T, Harizaj A, Steene SV, De Beer T, De Smedt S, Szunerits S, Boukherroub R, Xiong R, Braeckmans K. Liu J, et al. Among authors: brans t. Int J Mol Sci. 2020 Feb 24;21(4):1540. doi: 10.3390/ijms21041540. Int J Mol Sci. 2020. PMID: 32102402 Free PMC article.
34 results