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Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering.
Raes L, Pille M, Harizaj A, Goetgeluk G, Van Hoeck J, Stremersch S, Fraire JC, Brans T, de Jong OG, Maas-Bakker R, Mastrobattista E, Vader P, De Smedt SC, Vandekerckhove B, Raemdonck K, Braeckmans K. Raes L, et al. Among authors: brans t. Mol Ther Nucleic Acids. 2021 Aug 19;25:696-707. doi: 10.1016/j.omtn.2021.08.014. eCollection 2021 Sep 3. Mol Ther Nucleic Acids. 2021. PMID: 34589287 Free PMC article.
Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells.
Fraire JC, Shaabani E, Sharifiaghdam M, Rombaut M, Hinnekens C, Hua D, Ramon J, Raes L, Bolea-Fernandez E, Brans T, Vanhaecke F, Borghgraef P, Huang C, Sauvage F, Vanhaecke T, De Kock J, Xiong R, De Smedt S, Braeckmans K. Fraire JC, et al. Among authors: brans t. Nat Commun. 2022 Apr 14;13(1):1996. doi: 10.1038/s41467-022-29713-7. Nat Commun. 2022. PMID: 35422038 Free PMC article.
Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy.
Liu J, Xiong R, Brans T, Lippens S, Parthoens E, Zanacchi FC, Magrassi R, Singh SK, Kurungot S, Szunerits S, Bové H, Ameloot M, Fraire JC, Teirlinck E, Samal SK, Rycke R, Houthaeve G, De Smedt SC, Boukherroub R, Braeckmans K. Liu J, et al. Among authors: brans t. Light Sci Appl. 2018 Aug 8;7:47. doi: 10.1038/s41377-018-0048-3. eCollection 2018. Light Sci Appl. 2018. PMID: 30839577 Free PMC article.
Photoablation of Human Vitreous Opacities by Light-Induced Vapor Nanobubbles.
Sauvage F, Fraire JC, Remaut K, Sebag J, Peynshaert K, Harrington M, Van de Velde FJ, Xiong R, Tassignon MJ, Brans T, Braeckmans K, De Smedt SC. Sauvage F, et al. Among authors: brans t. ACS Nano. 2019 Jul 23;13(7):8401-8416. doi: 10.1021/acsnano.9b04050. Epub 2019 Jul 12. ACS Nano. 2019. PMID: 31287662
Vapor nanobubble is the more reliable photothermal mechanism for inducing endosomal escape of siRNA without disturbing cell homeostasis.
Fraire JC, Houthaeve G, Liu J, Raes L, Vermeulen L, Stremersch S, Brans T, García-Díaz Barriga G, De Keulenaer S, Van Nieuwerburgh F, De Rycke R, Vandesompele J, Mestdagh P, Raemdonck K, De Vos WH, De Smedt S, Braeckmans K. Fraire JC, et al. Among authors: brans t. J Control Release. 2020 Mar 10;319:262-275. doi: 10.1016/j.jconrel.2019.12.050. Epub 2020 Jan 2. J Control Release. 2020. PMID: 31904400
Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation.
Liu J, Li C, Brans T, Harizaj A, Steene SV, De Beer T, De Smedt S, Szunerits S, Boukherroub R, Xiong R, Braeckmans K. Liu J, et al. Among authors: brans t. Int J Mol Sci. 2020 Feb 24;21(4):1540. doi: 10.3390/ijms21041540. Int J Mol Sci. 2020. PMID: 32102402 Free PMC article.
34 results