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Hepatitis C virus E2 envelope glycoprotein produced in Nicotiana benthamiana triggers humoral response with virus-neutralizing activity in vaccinated mice.
Dobrica MO, van Eerde A, Tucureanu C, Onu A, Paruch L, Caras I, Vlase E, Steen H, Haugslien S, Alonzi D, Zitzmann N, Bock R, Dubuisson J, Popescu CI, Stavaru C, Liu Clarke J, Branza-Nichita N. Dobrica MO, et al. Among authors: liu clarke j. Plant Biotechnol J. 2021 Oct;19(10):2027-2039. doi: 10.1111/pbi.13631. Epub 2021 Jun 3. Plant Biotechnol J. 2021. PMID: 34002936 Free PMC article.
In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.
van Eerde A, Várnai A, Jameson JK, Paruch L, Moen A, Anonsen JH, Chylenski P, Steen HS, Heldal I, Bock R, Eijsink VGH, Liu-Clarke J. van Eerde A, et al. Among authors: liu clarke j. Plant Biotechnol J. 2020 Mar;18(3):631-643. doi: 10.1111/pbi.13227. Epub 2019 Aug 17. Plant Biotechnol J. 2020. PMID: 31373133 Free PMC article.
The "humanized" N-glycosylation pathway in CRISPR/Cas9-edited Nicotiana benthamiana significantly enhances the immunogenicity of a S/preS1 Hepatitis B Virus antigen and the virus-neutralizing antibody response in vaccinated mice.
Pantazica AM, van Eerde A, Dobrica MO, Caras I, Ionescu I, Costache A, Tucureanu C, Steen H, Lazar C, Heldal I, Haugslien S, Onu A, Stavaru C, Branza-Nichita N, Liu Clarke J. Pantazica AM, et al. Among authors: liu clarke j. Plant Biotechnol J. 2023 Jun;21(6):1176-1190. doi: 10.1111/pbi.14028. Epub 2023 Mar 10. Plant Biotechnol J. 2023. PMID: 36779605 Free PMC article.