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Current and future challenges in quality assurance in molecular diagnostics.
Ahmad-Nejad P, Ashavaid T, Vacaflores Salinas A, Huggett J, Harris K, Linder MW, Baluchova K, Steimer W, Payne DA; IFCC Committee for Molecular Diagnostics (C-MD). Ahmad-Nejad P, et al. Among authors: huggett j. Clin Chim Acta. 2021 Aug;519:239-246. doi: 10.1016/j.cca.2021.05.004. Epub 2021 May 8. Clin Chim Acta. 2021. PMID: 33971158
Molecular diagnostics of SARS-CoV-2: Findings of an international survey.
Vacaflores Salinas A, Ashavaid T, Payne DA, Linder MW, Baluchova K, Pan S, Huggett J, Ahmad-Nejad P; IFCC Committee for Molecular Diagnostics C-MD. Vacaflores Salinas A, et al. Among authors: huggett j. Clin Chim Acta. 2022 Jun 1;531:237-242. doi: 10.1016/j.cca.2022.04.007. Epub 2022 Apr 9. Clin Chim Acta. 2022. PMID: 35413298 Free PMC article.
The Dangers of Using Cq to Quantify Nucleic Acid in Biological Samples: A Lesson From COVID-19.
Evans D, Cowen S, Kammel M, O'Sullivan DM, Stewart G, Grunert HP, Moran-Gilad J, Verwilt J, In J, Vandesompele J, Harris K, Hong KH, Storey N, Hingley-Wilson S, Dühring U, Bae YK, Foy CA, Braybrook J, Zeichhardt H, Huggett JF. Evans D, et al. Among authors: huggett jf. Clin Chem. 2021 Dec 30;68(1):153-162. doi: 10.1093/clinchem/hvab219. Clin Chem. 2021. PMID: 34633030
Assessing the accuracy of quantitative molecular microbial profiling.
O'Sullivan DM, Laver T, Temisak S, Redshaw N, Harris KA, Foy CA, Studholme DJ, Huggett JF. O'Sullivan DM, et al. Among authors: huggett jf. Int J Mol Sci. 2014 Nov 21;15(11):21476-91. doi: 10.3390/ijms151121476. Int J Mol Sci. 2014. PMID: 25421243 Free PMC article.
The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis.
Devonshire AS, O'Sullivan DM, Honeyborne I, Jones G, Karczmarczyk M, Pavšič J, Gutteridge A, Milavec M, Mendoza P, Schimmel H, Van Heuverswyn F, Gorton R, Cirillo DM, Borroni E, Harris K, Barnard M, Heydenrych A, Ndusilo N, Wallis CL, Pillay K, Barry T, Reddington K, Richter E, Mozioğlu E, Akyürek S, Yalçınkaya B, Akgoz M, Žel J, Foy CA, McHugh TD, Huggett JF. Devonshire AS, et al. Among authors: huggett jf. BMC Infect Dis. 2016 Aug 3;16:366. doi: 10.1186/s12879-016-1696-7. BMC Infect Dis. 2016. PMID: 27487852 Free PMC article.
An inter-laboratory study to investigate the impact of the bioinformatics component on microbiome analysis using mock communities.
O'Sullivan DM, Doyle RM, Temisak S, Redshaw N, Whale AS, Logan G, Huang J, Fischer N, Amos GCA, Preston MD, Marchesi JR, Wagner J, Parkhill J, Motro Y, Denise H, Finn RD, Harris KA, Kay GL, O'Grady J, Ransom-Jones E, Wu H, Laing E, Studholme DJ, Benavente ED, Phelan J, Clark TG, Moran-Gilad J, Huggett JF. O'Sullivan DM, et al. Among authors: huggett jf. Sci Rep. 2021 May 19;11(1):10590. doi: 10.1038/s41598-021-89881-2. Sci Rep. 2021. PMID: 34012005 Free PMC article.
Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study.
Doyle RM, O'Sullivan DM, Aller SD, Bruchmann S, Clark T, Coello Pelegrin A, Cormican M, Diez Benavente E, Ellington MJ, McGrath E, Motro Y, Phuong Thuy Nguyen T, Phelan J, Shaw LP, Stabler RA, van Belkum A, van Dorp L, Woodford N, Moran-Gilad J, Huggett JF, Harris KA. Doyle RM, et al. Among authors: huggett jf. Microb Genom. 2020 Feb;6(2):e000335. doi: 10.1099/mgen.0.000335. Epub 2020 Feb 12. Microb Genom. 2020. PMID: 32048983 Free PMC article.
142 results