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RNA reference materials with defined viral RNA loads of SARS-CoV-2-A useful tool towards a better PCR assay harmonization.
Vierbaum L, Wojtalewicz N, Grunert HP, Lindig V, Duehring U, Drosten C, Corman V, Niemeyer D, Ciesek S, Rabenau HF, Berger A, Obermeier M, Nitsche A, Michel J, Mielke M, Huggett J, O'Sullivan D, Busby E, Cowen S, Vallone PM, Cleveland MH, Falak S, Kummrow A, Keller T, Schellenberg I, Zeichhardt H, Kammel M. Vierbaum L, et al. PLoS One. 2022 Jan 20;17(1):e0262656. doi: 10.1371/journal.pone.0262656. eCollection 2022. PLoS One. 2022. PMID: 35051208 Free PMC article.
The performance of human cytomegalovirus digital PCR reference measurement procedure in seven external quality assessment schemes over four years.
Milavec M, Pavšič J, Bogožalec Košir A, Jones GM, O'Sullivan DM, Devonshire AS, Van Heuverswyn F, Karczmarczyk M, Neeb J, Plauth A, Corbisier P, Schimmel H, Kummrow A, Neukammer J, Foy CA, Kammel M, Grunert HP, Zeichhardt H, Huggett JF. Milavec M, et al. Among authors: huggett jf. Methods. 2022 May;201:65-73. doi: 10.1016/j.ymeth.2021.03.016. Epub 2021 Apr 1. Methods. 2022. PMID: 33812016 Free article.
Digital PCR can augment the interpretation of RT-qPCR Cq values for SARS-CoV-2 diagnostics.
Whale AS, von der Heide EK, Kohlenberg M, Brinckmann A, Baedker S, Karalay O, Fernandez-Gonzalez A, Busby EJ, Bustin SA, Hauser H, Missel A, O'Sullivan DM, Huggett JF, Pfaffl MW, Nolan T. Whale AS, et al. Among authors: huggett jf. Methods. 2022 May;201:5-14. doi: 10.1016/j.ymeth.2021.08.006. Epub 2021 Aug 26. Methods. 2022. PMID: 34454016 Free PMC article.
The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis.
Devonshire AS, O'Sullivan DM, Honeyborne I, Jones G, Karczmarczyk M, Pavšič J, Gutteridge A, Milavec M, Mendoza P, Schimmel H, Van Heuverswyn F, Gorton R, Cirillo DM, Borroni E, Harris K, Barnard M, Heydenrych A, Ndusilo N, Wallis CL, Pillay K, Barry T, Reddington K, Richter E, Mozioğlu E, Akyürek S, Yalçınkaya B, Akgoz M, Žel J, Foy CA, McHugh TD, Huggett JF. Devonshire AS, et al. Among authors: huggett jf. BMC Infect Dis. 2016 Aug 3;16:366. doi: 10.1186/s12879-016-1696-7. BMC Infect Dis. 2016. PMID: 27487852 Free PMC article.
The Dangers of Using Cq to Quantify Nucleic Acid in Biological Samples: A Lesson From COVID-19.
Evans D, Cowen S, Kammel M, O'Sullivan DM, Stewart G, Grunert HP, Moran-Gilad J, Verwilt J, In J, Vandesompele J, Harris K, Hong KH, Storey N, Hingley-Wilson S, Dühring U, Bae YK, Foy CA, Braybrook J, Zeichhardt H, Huggett JF. Evans D, et al. Among authors: huggett jf. Clin Chem. 2021 Dec 30;68(1):153-162. doi: 10.1093/clinchem/hvab219. Clin Chem. 2021. PMID: 34633030
Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA.
Pavšič J, Devonshire A, Blejec A, Foy CA, Van Heuverswyn F, Jones GM, Schimmel H, Žel J, Huggett JF, Redshaw N, Karczmarczyk M, Mozioğlu E, Akyürek S, Akgöz M, Milavec M. Pavšič J, et al. Among authors: huggett jf. Anal Bioanal Chem. 2017 Apr;409(10):2601-2614. doi: 10.1007/s00216-017-0206-0. Epub 2017 Jan 26. Anal Bioanal Chem. 2017. PMID: 28124757 Free PMC article.
Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2.
Huggett JF, Benes V, Bustin SA, Garson JA, Harris K, Kammel M, Kubista M, McHugh TD, Moran-Gilad J, Nolan T, Pfaffl MW, Salit M, Shipley G, Vallone PM, Vandesompele J, Wittwer C, Zeichhardt H. Huggett JF, et al. Clin Chem. 2020 Nov 1;66(11):1369-1372. doi: 10.1093/clinchem/hvaa214. Clin Chem. 2020. PMID: 32894756 Free PMC article.
126 results