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Parallel PRC2/cPRC1 and vPRC1 pathways silence lineage-specific genes and maintain self-renewal in mouse embryonic stem cells.
Zepeda-Martinez JA, Pribitzer C, Wang J, Bsteh D, Golumbeanu S, Zhao Q, Burkard TR, Reichholf B, Rhie SK, Jude J, Moussa HF, Zuber J, Bell O. Zepeda-Martinez JA, et al. Among authors: burkard tr. Sci Adv. 2020 Apr 1;6(14):eaax5692. doi: 10.1126/sciadv.aax5692. eCollection 2020 Apr. Sci Adv. 2020. PMID: 32270030 Free PMC article.
Uridylation of RNA Hairpins by Tailor Confines the Emergence of MicroRNAs in Drosophila.
Reimão-Pinto MM, Ignatova V, Burkard TR, Hung JH, Manzenreither RA, Sowemimo I, Herzog VA, Reichholf B, Fariña-Lopez S, Ameres SL. Reimão-Pinto MM, et al. Among authors: burkard tr. Mol Cell. 2015 Jul 16;59(2):203-16. doi: 10.1016/j.molcel.2015.05.033. Epub 2015 Jul 2. Mol Cell. 2015. PMID: 26145176 Free PMC article.
Thiol-linked alkylation of RNA to assess expression dynamics.
Herzog VA, Reichholf B, Neumann T, Rescheneder P, Bhat P, Burkard TR, Wlotzka W, von Haeseler A, Zuber J, Ameres SL. Herzog VA, et al. Among authors: burkard tr. Nat Methods. 2017 Dec;14(12):1198-1204. doi: 10.1038/nmeth.4435. Epub 2017 Sep 25. Nat Methods. 2017. PMID: 28945705 Free PMC article.
A reversible haploid mouse embryonic stem cell biobank resource for functional genomics.
Elling U, Wimmer RA, Leibbrandt A, Burkard T, Michlits G, Leopoldi A, Micheler T, Abdeen D, Zhuk S, Aspalter IM, Handl C, Liebergesell J, Hubmann M, Husa AM, Kinzer M, Schuller N, Wetzel E, van de Loo N, Martinez JAZ, Estoppey D, Riedl R, Yang F, Fu B, Dechat T, Ivics Z, Agu CA, Bell O, Blaas D, Gerhardt H, Hoepfner D, Stark A, Penninger JM. Elling U, et al. Nature. 2017 Oct 5;550(7674):114-118. doi: 10.1038/nature24027. Epub 2017 Sep 27. Nature. 2017. PMID: 28953874 Free PMC article.
Multilayered VBC score predicts sgRNAs that efficiently generate loss-of-function alleles.
Michlits G, Jude J, Hinterndorfer M, de Almeida M, Vainorius G, Hubmann M, Neumann T, Schleiffer A, Burkard TR, Fellner M, Gijsbertsen M, Traunbauer A, Zuber J, Elling U. Michlits G, et al. Among authors: burkard tr. Nat Methods. 2020 Jul;17(7):708-716. doi: 10.1038/s41592-020-0850-8. Epub 2020 Jun 8. Nat Methods. 2020. PMID: 32514112
Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters.
Umkehrer C, Holstein F, Formenti L, Jude J, Froussios K, Neumann T, Cronin SM, Haas L, Lipp JJ, Burkard TR, Fellner M, Wiesner T, Zuber J, Obenauf AC. Umkehrer C, et al. Among authors: burkard tr. Nat Biotechnol. 2021 Feb;39(2):174-178. doi: 10.1038/s41587-020-0614-0. Epub 2020 Jul 27. Nat Biotechnol. 2021. PMID: 32719478
45 results