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New 20-hydroxycholesterol-like compounds with fluorescent NBD or alkyne labels: Synthesis, in silico interactions with proteins and uptake by yeast cells.
Chem Phys Lipids. 2020 Mar;227:104850. doi: 10.1016/j.chemphyslip.2019.104850. Epub 2019 Dec 10.
Chem Phys Lipids. 2020.
PMID: 31836520
Analysis of In Vivo Activity of the Bovine Cholesterol Hydroxylase/Lyase System Proteins Expressed in Escherichia coli.
Efimova VS, Isaeva LV, Rubtsov MA, Novikova LA.
Efimova VS, et al.
Mol Biotechnol. 2019 Apr;61(4):261-273. doi: 10.1007/s12033-019-00158-6.
Mol Biotechnol. 2019.
PMID: 30729436
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Expression of Cholesterol Hydroxylase/Lyase System Proteins in Yeast S. cerevisiae Cells as a Self-Processing Polyprotein.
Efimova VS, Isaeva LV, Makeeva DS, Rubtsov MA, Novikova LA.
Efimova VS, et al.
Mol Biotechnol. 2017 Oct;59(9-10):394-406. doi: 10.1007/s12033-017-0028-5.
Mol Biotechnol. 2017.
PMID: 28799023
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Ile351, Leu355 and Ile461 residues are essential for catalytic activity of bovine cytochrome P450scc (CYP11A1).
Glyakina AV, Strizhov NI, Karpov MV, Dovidchenko NV, Matkarimov BT, Isaeva LV, Efimova VS, Rubtsov MA, Novikova LA, Donova MV, Galzitskaya OV.
Glyakina AV, et al. Among authors: efimova vs.
Steroids. 2019 Mar;143:80-90. doi: 10.1016/j.steroids.2019.01.002. Epub 2019 Jan 11.
Steroids. 2019.
PMID: 30641046
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Using a viral 2A peptide-based strategy to reconstruct the bovine P450scc steroidogenic system in S. cerevisiae: Bovine P450scc system expression using 2A peptides.
Efimova VS, Isaeva LV, Orekhov PS, Bozdaganyan ME, Rubtsov MA, Novikova LA.
Efimova VS, et al.
J Biotechnol. 2021 Jan 10;325:186-195. doi: 10.1016/j.jbiotec.2020.10.028. Epub 2020 Nov 3.
J Biotechnol. 2021.
PMID: 33157198
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Polycistronic expression of the mitochondrial steroidogenic P450scc system in the HEK293T cell line.
Efimova VS, Isaeva LV, Labudina AA, Tashlitsky VN, Rubtsov MA, Novikova LA.
Efimova VS, et al.
J Cell Biochem. 2019 Mar;120(3):3124-3136. doi: 10.1002/jcb.27577. Epub 2018 Sep 11.
J Cell Biochem. 2019.
PMID: 30272820
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