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Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli.
Sequeira AF, Turchetto J, Saez NJ, Peysson F, Ramond L, Duhoo Y, Blémont M, Fernandes VO, Gama LT, Ferreira LM, Guerreiro CI, Gilles N, Darbon H, Fontes CM, Vincentelli R. Sequeira AF, et al. Among authors: gilles n. Microb Cell Fact. 2017 Jan 17;16(1):4. doi: 10.1186/s12934-016-0618-0. Microb Cell Fact. 2017. PMID: 28093085 Free PMC article.
High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery.
Turchetto J, Sequeira AF, Ramond L, Peysson F, Brás JL, Saez NJ, Duhoo Y, Blémont M, Guerreiro CI, Quinton L, De Pauw E, Gilles N, Darbon H, Fontes CM, Vincentelli R. Turchetto J, et al. Among authors: gilles n. Microb Cell Fact. 2017 Jan 17;16(1):6. doi: 10.1186/s12934-016-0617-1. Microb Cell Fact. 2017. PMID: 28095880 Free PMC article.
High-Throughput Production of Oxidized Animal Toxins in Escherichia coli.
Duhoo Y, Sequeira AF, Saez NJ, Turchetto J, Ramond L, Peysson F, Brás JLA, Gilles N, Darbon H, Fontes CMGA, Vincentelli R. Duhoo Y, et al. Among authors: gilles n. Methods Mol Biol. 2019;2025:165-190. doi: 10.1007/978-1-4939-9624-7_7. Methods Mol Biol. 2019. PMID: 31267452
A Venomics Approach Coupled to High-Throughput Toxin Production Strategies Identifies the First Venom-Derived Melanocortin Receptor Agonists.
Reynaud S, Ciolek J, Degueldre M, Saez NJ, Sequeira AF, Duhoo Y, Brás JLA, Meudal H, Cabo Díez M, Fernández Pedrosa V, Verdenaud M, Boeri J, Pereira Ramos O, Ducancel F, Vanden Driessche M, Fourmy R, Violette A, Upert G, Mourier G, Beck-Sickinger AG, Mörl K, Landon C, Fontes CMGA, Miñambres Herráiz R, Rodríguez de la Vega RC, Peigneur S, Tytgat J, Quinton L, De Pauw E, Vincentelli R, Servent D, Gilles N. Reynaud S, et al. Among authors: gilles n. J Med Chem. 2020 Aug 13;63(15):8250-8264. doi: 10.1021/acs.jmedchem.0c00485. Epub 2020 Jul 16. J Med Chem. 2020. PMID: 32602722
High-throughput production of two disulphide-bridge toxins.
Upert G, Mourier G, Pastor A, Verdenaud M, Alili D, Servent D, Gilles N. Upert G, et al. Among authors: gilles n. Chem Commun (Camb). 2014 Aug 7;50(61):8408-11. doi: 10.1039/c4cc02679a. Chem Commun (Camb). 2014. PMID: 24947561
Crystallization of recombinant green mamba ρ-Da1a toxin during a lyophilization procedure and its structure determination.
Maïga A, Vera L, Marchetti C, Lorphelin A, Bellanger L, Mourier G, Servent D, Gilles N, Stura EA. Maïga A, et al. Among authors: gilles n. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jun;69(Pt 6):704-9. doi: 10.1107/S1744309113011470. Epub 2013 May 29. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013. PMID: 23722859 Free PMC article.
The European FP7 Venomics Project.
Gilles N, Servent D. Gilles N, et al. Future Med Chem. 2014 Oct;6(15):1611-2. doi: 10.4155/fmc.14.85. Future Med Chem. 2014. PMID: 25406001 No abstract available.
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