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DNA binding specificity of MunI restriction endonuclease is controlled by pH and calcium ions: involvement of active site carboxylate residues.
Lagunavicius A, Grazulis S, Balciunaite E, Vainius D, Siksnys V. Lagunavicius A, et al. Among authors: siksnys v. Biochemistry. 1997 Sep 16;36(37):11093-9. doi: 10.1021/bi963126a. Biochemistry. 1997. PMID: 9287152
Gel shift analysis reveals [Lagunavicius, A., & Siksnys, V. (1997) Biochemistry 36 (preceding paper in this issue)] that at pH 8.3 in the absence of Mg2+, MunI restriction endonuclease exhibits little DNA binding specificity, as compared with the D83A and E98A m …
Gel shift analysis reveals [Lagunavicius, A., & Siksnys, V. (1997) Biochemistry 36 (preceding paper in this issue)] that a …
Tetrameric restriction enzymes: expansion to the GIY-YIG nuclease family.
Gasiunas G, Sasnauskas G, Tamulaitis G, Urbanke C, Razaniene D, Siksnys V. Gasiunas G, et al. Among authors: siksnys v. Nucleic Acids Res. 2008 Feb;36(3):938-49. doi: 10.1093/nar/gkm1090. Epub 2007 Dec 17. Nucleic Acids Res. 2008. PMID: 18086711 Free PMC article.
Molecular mechanisms of CRISPR-mediated microbial immunity.
Gasiunas G, Sinkunas T, Siksnys V. Gasiunas G, et al. Among authors: siksnys v. Cell Mol Life Sci. 2014 Feb;71(3):449-65. doi: 10.1007/s00018-013-1438-6. Cell Mol Life Sci. 2014. PMID: 23959171 Free PMC article. Review.
133 results