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Assignment of function to histidines 260 and 298 by engineering the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase complex; substitutions that lead to acceptance of substrates lacking the 5-carboxyl group.
Shim da J, Nemeria NS, Balakrishnan A, Patel H, Song J, Wang J, Jordan F, Farinas ET. Shim da J, et al. Among authors: wang j. Biochemistry. 2011 Sep 6;50(35):7705-9. doi: 10.1021/bi200936n. Epub 2011 Aug 10. Biochemistry. 2011. PMID: 21809826 Free PMC article.
Novel binding motif and new flexibility revealed by structural analyses of a pyruvate dehydrogenase-dihydrolipoyl acetyltransferase subcomplex from the Escherichia coli pyruvate dehydrogenase multienzyme complex.
Arjunan P, Wang J, Nemeria NS, Reynolds S, Brown I, Chandrasekhar K, Calero G, Jordan F, Furey W. Arjunan P, et al. Among authors: wang j. J Biol Chem. 2014 Oct 24;289(43):30161-76. doi: 10.1074/jbc.M114.592915. Epub 2014 Sep 10. J Biol Chem. 2014. PMID: 25210042 Free PMC article.
Insight to the interaction of the dihydrolipoamide acetyltransferase (E2) core with the peripheral components in the Escherichia coli pyruvate dehydrogenase complex via multifaceted structural approaches.
Chandrasekhar K, Wang J, Arjunan P, Sax M, Park YH, Nemeria NS, Kumaran S, Song J, Jordan F, Furey W. Chandrasekhar K, et al. Among authors: wang j. J Biol Chem. 2013 May 24;288(21):15402-17. doi: 10.1074/jbc.M113.466789. Epub 2013 Apr 11. J Biol Chem. 2013. PMID: 23580650 Free PMC article.
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