Improved thermostability of Clostridium thermocellum endoglucanase Cel8A by using consensus-guided mutagenesis.
Anbar M, Gul O, Lamed R, Sezerman UO, Bayer EA.
Anbar M, et al.
Appl Environ Microbiol. 2012 May;78(9):3458-64. doi: 10.1128/AEM.07985-11. Epub 2012 Mar 2.
Appl Environ Microbiol. 2012.
PMID: 22389377
Free PMC article.
Previously, we have demonstrated the engineering of an enhanced thermostable family 8 cellulosomal endoglucanase (EC 3.2.1.4), Cel8A, from Clostridium thermocellum, using random error-prone PCR and a combination of three beneficial mutations, dominated by an intriguing serine-to- …
Previously, we have demonstrated the engineering of an enhanced thermostable family 8 cellulosomal endoglucanase (EC 3.2.1.4), Cel8A, from C …