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Sequential hydroxylation of vitamin D2 by a genetically engineered CYP105A1.
Hayashi K, Yasuda K, Yogo Y, Takita T, Yasukawa K, Ohta M, Kamakura M, Ikushiro S, Sakaki T. Hayashi K, et al. Among authors: kamakura m. Biochem Biophys Res Commun. 2016 May 13;473(4):853-858. doi: 10.1016/j.bbrc.2016.03.139. Epub 2016 Mar 30. Biochem Biophys Res Commun. 2016. PMID: 27037023
Production of an active form of vitamin D2 by genetically engineered CYP105A1.
Yasuda K, Yogo Y, Sugimoto H, Mano H, Takita T, Ohta M, Kamakura M, Ikushiro S, Yasukawa K, Shiro Y, Sakaki T. Yasuda K, et al. Among authors: kamakura m. Biochem Biophys Res Commun. 2017 Apr 29;486(2):336-341. doi: 10.1016/j.bbrc.2017.03.040. Epub 2017 Mar 14. Biochem Biophys Res Commun. 2017. PMID: 28302483
Protein engineering of CYP105s for their industrial uses.
Yasuda K, Sugimoto H, Hayashi K, Takita T, Yasukawa K, Ohta M, Kamakura M, Ikushiro S, Shiro Y, Sakaki T. Yasuda K, et al. Among authors: kamakura m. Biochim Biophys Acta Proteins Proteom. 2018 Jan;1866(1):23-31. doi: 10.1016/j.bbapap.2017.05.014. Epub 2017 Jun 2. Biochim Biophys Acta Proteins Proteom. 2018. PMID: 28583351 Review.
Structure-function analysis of vitamin D 24-hydroxylase (CYP24A1) by site-directed mutagenesis: amino acid residues responsible for species-based difference of CYP24A1 between humans and rats.
Hamamoto H, Kusudo T, Urushino N, Masuno H, Yamamoto K, Yamada S, Kamakura M, Ohta M, Inouye K, Sakaki T. Hamamoto H, et al. Among authors: kamakura m. Mol Pharmacol. 2006 Jul;70(1):120-8. doi: 10.1124/mol.106.023275. Epub 2006 Apr 14. Mol Pharmacol. 2006. PMID: 16617161
Metabolism of 1alpha,25-dihydroxyvitamin D2 by human CYP24A1.
Urushino N, Yasuda K, Ikushiro S, Kamakura M, Ohta M, Sakaki T. Urushino N, et al. Among authors: kamakura m. Biochem Biophys Res Commun. 2009 Jun 26;384(2):144-8. doi: 10.1016/j.bbrc.2009.04.075. Epub 2009 Apr 23. Biochem Biophys Res Commun. 2009. PMID: 19393625
132 results