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In vivo packaging of brome mosaic virus RNA3, but not RNAs 1 and 2, is dependent on a cis-acting 3' tRNA-like structure.
Annamalai P, Rao AL. Annamalai P, et al. J Virol. 2007 Jan;81(1):173-81. doi: 10.1128/JVI.01500-06. Epub 2006 Sep 27. J Virol. 2007. PMID: 17005656 Free PMC article.
To validate these observations in vivo, we performed packaging assays using Agrobacterium-mediated transient expression of RNAs and coat protein (CP) (P. Annamalai and A. L. N. Rao, Virology 338:96-111, 2005). Coexpression of TLS-less constructs of B1 or B2 or B3 an …
To validate these observations in vivo, we performed packaging assays using Agrobacterium-mediated transient expression of RNAs and coat pro …
Packaging of brome mosaic virus subgenomic RNA is functionally coupled to replication-dependent transcription and translation of coat protein.
Annamalai P, Rao AL. Annamalai P, et al. J Virol. 2006 Oct;80(20):10096-108. doi: 10.1128/JVI.01186-06. J Virol. 2006. PMID: 17005687 Free PMC article.
To confirm these observations in vivo and to unravel the mechanism of sgB4 copackaging, an Agrobacterium-mediated transient in vivo expression system (P. Annamalai and A. L. N. Rao, Virology 338:96-111, 2005) that effectively uncouples replication from packaging was …
To confirm these observations in vivo and to unravel the mechanism of sgB4 copackaging, an Agrobacterium-mediated transient in vivo expressi …
RNA encapsidation assay.
Annamalai P, Rao AL. Annamalai P, et al. Methods Mol Biol. 2008;451:251-64. doi: 10.1007/978-1-59745-102-4_18. Methods Mol Biol. 2008. PMID: 18370261
68 results