Involvement of the "occluded nucleotide conformation" of P-glycoprotein in the catalytic pathway.
Tombline G, Muharemagić A, White LB, Senior AE.
Tombline G, et al.
Biochemistry. 2005 Sep 27;44(38):12879-86. doi: 10.1021/bi0509797.
Biochemistry. 2005.
PMID: 16171403
We found recently that the combined mutation of both "catalytic carboxylate" residues (E552A/E1197A) in mouse P-glycoprotein (Pgp) arrested the protein in an "occluded nucleotide conformation", possibly a stabilized dimer of nucleotide-binding domains (NBDs), that binds MgATP tig …
We found recently that the combined mutation of both "catalytic carboxylate" residues (E552A/E1197A) in mouse P-glycoprotein (Pgp) arrested …