Abstract
We report the cloning of both the cDNA and the corresponding genomic sequence of a new PP2C from Arabidopsis thaliana, named AtP2C-HA (for homology to ABI1/ABI2). The AtP2C-HA cDNA contains an open reading frame of 1536 bp and encodes a putative protein of 511 amino acids with a predicted molecular mass of 55.7 kDa. The AtP2C-HA protein is composed of two domains, a C-terminal PP2C catalytic domain and a N-terminal extension of ca. 180 amino acid residues. The deduced amino acid sequence is 55% and 54% identical to ABI1 and ABI2, respectively. Comparison of the genomic structure of the ABI1, ABI2 and AtP2C-HA genes suggests that they belong to a multigene family. The expression of the AtP2C-HA gene is up-regulated by abscisic acid (ABA) treatment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / pharmacology
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Amino Acid Sequence
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Arabidopsis / chemistry
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Arabidopsis / enzymology
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Arabidopsis / genetics*
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Arabidopsis Proteins*
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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DNA, Plant / chemistry
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DNA, Plant / genetics
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Exons
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Plant / drug effects
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Genes, Plant / genetics
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Introns
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Molecular Sequence Data
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Phosphoprotein Phosphatases / genetics*
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Protein Phosphatase 2
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Protein Phosphatase 2C
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RNA, Messenger / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Tissue Distribution
Substances
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Arabidopsis Proteins
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DNA, Complementary
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DNA, Plant
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P2C-HA protein, Arabidopsis
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RNA, Messenger
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Saccharomyces cerevisiae Proteins
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Abscisic Acid
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ABI1 protein, Arabidopsis
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ABI2 protein, Arabidopsis
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PTC1 protein, S cerevisiae
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Phosphoprotein Phosphatases
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Protein Phosphatase 2
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Protein Phosphatase 2C