Abstract
Graded activity of the DPP signaling pathway is critical for specification of dorsal embryonic cell fates in Drosophila. We present evidence that a second BMP ligand, SCW, potentiates DPP activity. Using dominant-negative forms of the type I receptors SAX and TKV, we demonstrate that SAX mediates the SCW signal, while TKV is required for both DPP and SCW activity. We find that while DPP/TKV signaling is obligatorily required, SCW/SAX activity is necessary but not sufficient for dorsal patterning. SAX and TKV act synergistically, suggesting a mechanism for integration of the SCW and DPP signals. Further, we show that the extracellular protein SOG can antagonize SCW, thus limiting its ability to augment DPP signaling in a graded manner.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Body Patterning / physiology
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Bone Morphogenetic Proteins / metabolism
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Bone Morphogenetic Proteins / physiology*
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Dimerization
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Drosophila / embryology*
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Drosophila Proteins*
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Drug Synergism
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Insect Proteins / physiology
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Ligands
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Protein Serine-Threonine Kinases / physiology*
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Receptors, Cell Surface / physiology*
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Receptors, Transforming Growth Factor beta / physiology*
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Signal Transduction / physiology*
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Transforming Growth Factor beta / metabolism
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Transforming Growth Factor beta / physiology*
Substances
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Bone Morphogenetic Proteins
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Drosophila Proteins
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Insect Proteins
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Ligands
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Receptors, Cell Surface
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Receptors, Transforming Growth Factor beta
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SCW protein, Drosophila
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Transforming Growth Factor beta
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dpp protein, Drosophila
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sax protein, Drosophila
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sog protein, Drosophila
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tkv protein, Drosophila
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Protein Serine-Threonine Kinases