Abstract
Using in vivo translational gene fusion in Escherichia coli K-12 we identified a gene that is specifically induced by heavy metals, cadmium, mercury and zinc, at nmolar concentrations. This gene was identified by homology to known zinc and cadmium transporters. We created a disruption of the gene that resulted only in a minor increase in sensitivity to cadmium, suggesting that the fusion, which is at the carboxy-terminal end of the molecule, probably allows for at least partial activity of the protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / genetics*
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Artificial Gene Fusion
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Cadmium / metabolism
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Cadmium / pharmacology
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Escherichia coli / drug effects*
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Escherichia coli / genetics*
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Escherichia coli / growth & development
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Gene Expression Regulation, Bacterial*
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Genes, Bacterial
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Heat-Shock Response / genetics
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Metals, Heavy / pharmacology*
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Oxidative Stress / genetics
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Protein Biosynthesis
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beta-Galactosidase / metabolism
Substances
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Metals, Heavy
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Cadmium
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beta-Galactosidase
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Adenosine Triphosphatases
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Zn(II)-translocating P-type ATPase