Attachment and growth of human rotaviruses RV-3 and S12/85 in Caco-2 cells depend on VP4

J Virol. 1998 Nov;72(11):9348-52. doi: 10.1128/JVI.72.11.9348-9352.1998.

Abstract

Studies with human neonatal rotaviruses RV-3 and S12/85 and their reassortants showed that VP4 is a determinant of rotavirus attachment to and growth in Caco-2 cells. The binding of these viruses to MA104 and Caco-2 cells correlated with their growth ability. Virus sensitivity to trypsin and the VP4 fusion region may be implicated in these processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Caco-2 Cells
  • Capsid / genetics
  • Capsid / physiology*
  • Capsid Proteins*
  • Cell Line
  • Genes, Viral
  • Humans
  • Infant, Newborn
  • Molecular Sequence Data
  • Rotavirus / genetics
  • Rotavirus / growth & development*
  • Rotavirus / pathogenicity*
  • Rotavirus Infections / etiology
  • Rotavirus Infections / virology
  • Sequence Homology, Amino Acid
  • Trypsin / pharmacology

Substances

  • Capsid Proteins
  • VP4 protein, Rotavirus
  • Trypsin

Associated data

  • GENBANK/AF076925
  • GENBANK/AF076926