Abstract
Desiccation of plants during drought can be detrimental to agricultural production. The phytohormone abscisic acid (ABA) reduces water loss by triggering stomatal pore closure in leaves, a process requiring ion-channel modulation by cytoplasmic proteins. Deletion of the Arabidopsis farnesyltransferase gene ERA1 or application of farnesyltransferase inhibitors resulted in ABA hypersensitivity of guard cell anion-channel activation and of stomatal closing. ERA1 was expressed in guard cells. Double-mutant analyses of era1 with the ABA-insensitive mutants abi1 and abi2 showed that era1 suppresses the ABA-insensitive phenotypes. Moreover, era1 plants exhibited a reduction in transpirational water loss during drought treatment.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Abscisic Acid / metabolism*
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Abscisic Acid / pharmacology
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Alkyl and Aryl Transferases / antagonists & inhibitors
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Alkyl and Aryl Transferases / genetics
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Alkyl and Aryl Transferases / metabolism*
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Anions
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Arabidopsis / cytology
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins*
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Enzyme Inhibitors / pharmacology
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Farnesol / analogs & derivatives
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Farnesol / pharmacology
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Gene Deletion
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Gene Expression
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Genes, Plant
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Ion Channels / metabolism*
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Mutation
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Organophosphonates / pharmacology
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Patch-Clamp Techniques
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Phosphoprotein Phosphatases / genetics
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Phosphoprotein Phosphatases / metabolism
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Plant Leaves / cytology
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Plant Leaves / genetics
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Plant Leaves / metabolism
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Plants, Genetically Modified
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Polyenes / pharmacology
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Polyunsaturated Alkamides
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Protein Prenylation
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Signal Transduction
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Water / metabolism*
Substances
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(alpha-hydroxyfarnesyl)phosphonic acid
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Anions
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Arabidopsis Proteins
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Enzyme Inhibitors
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Ion Channels
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Organophosphonates
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Polyenes
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Polyunsaturated Alkamides
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Water
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Farnesol
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Abscisic Acid
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Alkyl and Aryl Transferases
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p21(ras) farnesyl-protein transferase
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ABI1 protein, Arabidopsis
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ABI2 protein, Arabidopsis
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Phosphoprotein Phosphatases
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manumycin