This article describes regional variations in trabecular bone architecture in terms of density and orientation within six glenoid specimens. The mean donor age was 56 years and ranged from 31 to 72 years. An automated imaging technique based on 3-dimensional serial sectioning was used for the direct examination of the glenoid cancellous bone structures. Subchondral plate thickness was on average 1.9 mm and ranged from 1.2 mm to 2.9 mm. The volume fraction of trabecular bone varied from 11% to 45% with peak values at the posterior glenoid vault. On graphic 3-dimensional reconstructions, the glenoid appeared as platelike trabeculae, radially oriented perpendicular to the subchondral plate and interconnected by thin rods. These views also displayed regional variations throughout the glenoid, reflecting differences in the macroscopic appearance. Quantitative structural analysis revealed different degrees of anisotropy at the glenoid cancellous region, predominantly transverse isotropy. Resemblance to direct weight-bearing cancellous bone such as the proximal tibia was evident.