Abstract
Two recently described proteins in the mitochondrial uncoupling protein (UCP) family, UCP-2 and UCP-3, have been linked to phenotypes of obesity and NIDDM. We determined the mRNA levels of UCP-2 and UCP-3 in skeletal muscle of NIDDM patients and of healthy control subjects. No difference in the mRNA levels or in the protein expression of UCP-2 was observed between the two groups. In contrast, mRNA levels of UCP-3 were significantly reduced in skeletal muscle of NIDDM patients compared with control subjects. In the NIDDM patients, a positive correlation between UCP-3 expression and whole-body insulin-mediated glucose utilization rate was also noted. These results suggest that UCP-3 regulation may be altered in states of insulin resistance.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blood Glucose / metabolism
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Carrier Proteins / biosynthesis
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Carrier Proteins / genetics*
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DNA Primers
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Diabetes Mellitus, Type 2 / genetics
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Diabetes Mellitus, Type 2 / metabolism*
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Glucose / metabolism
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Glycated Hemoglobin / analysis
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Humans
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Insulin / blood
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Insulin / physiology
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Ion Channels
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Middle Aged
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Mitochondria, Muscle / metabolism
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Mitochondrial Proteins
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Muscle, Skeletal / metabolism*
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Polymerase Chain Reaction
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Protein Biosynthesis
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RNA, Messenger / metabolism
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Reference Values
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Transcription, Genetic
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Uncoupling Protein 3
Substances
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Blood Glucose
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Carrier Proteins
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DNA Primers
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Glycated Hemoglobin A
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Insulin
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Ion Channels
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Mitochondrial Proteins
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RNA, Messenger
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UCP3 protein, human
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Uncoupling Protein 3
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Glucose