Plant derived estrogens relax rat mesenteric artery in vitro

Abstract

The aim of this study was to investigate whether plant derived estrogens have the same relaxing effects in vitro as estradiol-17beta on arterial smooth muscle. The mesenteric arterial rings of female and male Wistar rats were studied. The relaxing effects of estradiol-17beta, genistein, daidzein and beta-sitosterol were determined, with particular focus on the role of endothelium. B-sitosterol had no relaxing effect on the arteries. Estradiol-17beta, genistein and daidzein relaxed noradrenaline, potassium chloride and calcium chloride precontracted arterial rings endothelium-independently. The relaxation responses were also independent of gender. Neither the removal of endothelium, nor the inhibition prostacyclin or nitric oxide synthesis, had any effect on the relaxation responses. The exact mechanism of these findings is still unclear.