Electrochemical detection of microcystins, cyanobacterial peptide hepatotoxins, following high-performance liquid chromatography

J Chromatogr A. 1998 Jun 12;810(1-2):226-30. doi: 10.1016/s0021-9673(98)00203-9.

Abstract

A novel amperometric HPLC detection method for the cyanobacterial (blue-green algal) peptide toxins microcystin-LR, -YR and -RR was developed. Purified microcystins and cyanobacterial extracts were chromatographed using an internal surface reversed-phase column with acetate- and phosphate-based mobile phase systems. Electrochemical oxidation reactions at 1.20 V vs. Ag/AgCl (glassy carbon working electrode) were show to originate in arginine and tyrosine residues of microcystins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Toxins / analysis*
  • Chemical and Drug Induced Liver Injury / metabolism*
  • Chromatography, High Pressure Liquid
  • Cyanobacteria / chemistry*
  • Electrochemistry
  • Indicators and Reagents
  • Microcystins
  • Peptides, Cyclic / analysis*
  • Spectrophotometry, Ultraviolet

Substances

  • Bacterial Toxins
  • Indicators and Reagents
  • Microcystins
  • Peptides, Cyclic
  • microcystin