Molecular cloning of rat Pax4: identification of four isoforms in rat insulinoma cells

Biochem Biophys Res Commun. 1998 Jul 9;248(1):153-6. doi: 10.1006/bbrc.1998.8925.

Abstract

Molecular cloning of rat Pax4 cDNA from a rat insulinoma cell line, RINm5F, library by PCR-based cloning strategy revealed four isoforms of the protein. Analysis of tissue distribution using Northern blotting and RT-PCR showed specific expression of Pax4 mRNA in pancreatic islets and RIN cells. RT-PCR confirmed that the mRNAs of four isoforms are expressed in RIN cells. These Pax4 variants may regulate the transcriptional activity of Pax4 during the development of pancreatic islets.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Cloning, Molecular
  • Homeodomain Proteins / chemistry*
  • Homeodomain Proteins / genetics*
  • Insulinoma / genetics*
  • Islets of Langerhans / chemistry*
  • Molecular Sequence Data
  • Paired Box Transcription Factors
  • Pancreatic Neoplasms / genetics*
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Rats
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics*
  • Tumor Cells, Cultured

Substances

  • Homeodomain Proteins
  • Paired Box Transcription Factors
  • Pax4 protein, rat
  • RNA, Messenger
  • Transcription Factors

Associated data

  • GENBANK/AF053100
  • GENBANK/AF053101
  • GENBANK/AF053102
  • GENBANK/AF053103