Purpose: In order to obtain the same characteristic tumor cells in genetical and biological aspect, the cloned human retinoblastoma cell line SO-Rb50 established in our department is reported in this paper.
Method: SO-Rb50 cells were cloned in multi-well plates.
Result: Non-rosette Rb cells were successfully cloned while rosette Rb cells failed.
Conclusion: The result suggested that high differentiated Rb cells were difficult to be cloned.