Differentiation of a porcine reproductive and respiratory syndrome virus vaccine strain from North American field strains by restriction fragment length polymorphism analysis of ORF 5

J Vet Diagn Invest. 1998 Apr;10(2):140-4. doi: 10.1177/104063879801000204.

Abstract

The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections.

MeSH terms

  • Animals
  • Base Sequence
  • Codon
  • DNA Restriction Enzymes
  • North America
  • Open Reading Frames*
  • Phylogeny
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length*
  • Porcine Reproductive and Respiratory Syndrome / immunology*
  • Porcine Reproductive and Respiratory Syndrome / prevention & control
  • Porcine respiratory and reproductive syndrome virus / classification
  • Porcine respiratory and reproductive syndrome virus / genetics*
  • Porcine respiratory and reproductive syndrome virus / immunology*
  • RNA, Viral / analysis
  • Swine
  • Viral Vaccines*

Substances

  • Codon
  • RNA, Viral
  • Viral Vaccines
  • DNA Restriction Enzymes

Associated data

  • GENBANK/AF020048
  • GENBANK/AF020049
  • GENBANK/AF020050