The gene downstream of Streptomyces aureofaciens whiB encodes a large protein with proposed transmembrane localization, and is induced by glucose

Biochim Biophys Acta. 1998 Apr 29;1397(2):151-5. doi: 10.1016/s0167-4781(98)00013-x.

Abstract

The sequence analysis of the region downstream of the Streptomyces aureofaciens whiB sporulation gene revealed a long open reading frame (1219 amino acids; Mr 128 209) encoding protein with potential transmembrane structure. By integrative transformation, via double cross-over, a stable null mutant of the gene, orf1219, was prepared. This mutation appeared to have no obvious effect on vegetative growth and differentiation. In vitro and in vivo transcriptional analysis of the downstream gene revealed a single apparent promoter induced by glucose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Glucose / pharmacology*
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Mutagenesis
  • Promoter Regions, Genetic
  • Streptomyces aureofaciens / genetics*
  • Transcription Factors / genetics

Substances

  • Bacterial Proteins
  • Membrane Proteins
  • Orf1219 protein, Streptomyces aureofaciens
  • Transcription Factors
  • whiB protein, Streptomyces
  • Glucose

Associated data

  • GENBANK/L22864