The sequence analysis of the region downstream of the Streptomyces aureofaciens whiB sporulation gene revealed a long open reading frame (1219 amino acids; Mr 128 209) encoding protein with potential transmembrane structure. By integrative transformation, via double cross-over, a stable null mutant of the gene, orf1219, was prepared. This mutation appeared to have no obvious effect on vegetative growth and differentiation. In vitro and in vivo transcriptional analysis of the downstream gene revealed a single apparent promoter induced by glucose.
Copyright 1998 Elsevier Science B.V.