Abstract
Precursor-accumulating (PAC) vesicles were previously shown to mediate the transport of the precursor of a major storage protein (pro2S albumin) to protein-storage vacuoles in developing pumpkin cotyledons. In this study, we characterized two homologous proteins from PAC vesicles, a 72 kDa protein (PV72) and an 82 kDa protein (PV82). PV72 and PV82 showed an ability to bind to peptides derived from both an internal propeptide and a C-terminal peptide of pro2S albumin. PV72 was predicted to be a type I integral membrane protein with epidermal growth factor (EGF)-like motifs. These results suggest that PV72 and PV82 are potential sorting receptors for 2S albumin to protein-storage vacuoles.
MeSH terms
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2S Albumins, Plant
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Albumins / metabolism
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Amino Acid Sequence
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Antigens, Plant
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Biological Transport
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Cucurbitaceae / genetics
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Cucurbitaceae / metabolism*
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DNA, Complementary
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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Protein Binding
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Protein Precursors / metabolism
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Receptors, Cytoplasmic and Nuclear / genetics
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Receptors, Cytoplasmic and Nuclear / metabolism*
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Sequence Homology, Amino Acid
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Vacuoles / metabolism
Substances
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2S Albumins, Plant
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Albumins
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Antigens, Plant
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DNA, Complementary
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Membrane Proteins
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PAC vesicle 72-kDa protein, Cucurbita
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Plant Proteins
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Protein Precursors
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Receptors, Cytoplasmic and Nuclear