Maize TF IIIA--the first transcription factor IIIA from monocotyledons. Purification and properties

Acta Biochim Pol. 1997;44(3):579-89.

Abstract

Purification and properties of transcription factor IIIA (TF IIIA) from maize pollen (Zea mays L.) are presented for the first time. The purified protein has a molecular mass of about 35 kDa and exhibits binding affinity toward both 5S rRNA and 5S rRNA gene. It also facilitates transcription of the 5S rRNA gene in a HeLa cell extract.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cotyledon / metabolism*
  • DNA-Binding Proteins / isolation & purification*
  • DNA-Binding Proteins / metabolism
  • HeLa Cells
  • Humans
  • Pollen / metabolism
  • RNA, Ribosomal, 5S / genetics
  • Transcription Factor TFIIIA
  • Transcription Factors / isolation & purification*
  • Transcription Factors / metabolism
  • Transcription, Genetic
  • Zea mays / metabolism*

Substances

  • DNA-Binding Proteins
  • RNA, Ribosomal, 5S
  • Transcription Factor TFIIIA
  • Transcription Factors